Literature DB >> 7417887

Specific binding of 2-[3H]chloroadenosine to rat brain cortical membranes.

P H Wu, J W Phillis, K Balls, B Rinaldi.   

Abstract

2-[3H]Chloroadenosine was used as a ligand to investigate the presence of adenosine receptors on rat brain cortical membranes. The binding studies reveal the presence of a single binding site which has an apparent dissociation constant (KD) of 23.5 nM and a maximal binding capacity (Bmax) of 476 fmol/mg protein. This binding can be inhibited by adenosine, adenine, nicotinamide adenine dinucleotide phosphate, inosine, theophylline, and isobutylmethylxanthine. These findings strongly support the suggestion that there is an adenosine receptor on brain cell membranes.

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Year:  1980        PMID： 7417887     DOI： 10.1139/y80-096

Source DB:  PubMed          Journal:  Can J Physiol Pharmacol        ISSN： 0008-4212            Impact factor:   2.273


  7 in total

1.  Biochemical characterization of putative central purinergic receptors by using 2-chloro[3H]adenosine, a stable analog of adenosine.

Authors:  M Williams; E A Risley
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

2.  Hypothermia-induced supersensitivity to adenosine for responses mediated via A1-receptors but not A2-receptors.

Authors:  K J Broadley; S Broome; D M Paton
Journal:  Br J Pharmacol       Date:  1985-02       Impact factor: 8.739

3.  The impact of inosine on hippocampal synaptic transmission and plasticity involves the release of adenosine through equilibrative nucleoside transporters rather than the direct activation of adenosine receptors.

Authors:  Pedro Valada; Sonja Hinz; Christin Vielmuth; Cátia R Lopes; Rodrigo A Cunha; Christa E Müller; João Pedro Lopes
Journal:  Purinergic Signal       Date:  2022-09-26       Impact factor: 3.950

4.  [3H]5'-N-ethylcarboxamide adenosine binds to both Ra and Ri adenosine receptors in rat striatum.

Authors:  S M Yeung; R D Green
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1984-03       Impact factor: 3.000

5.  Release of norepinephrine and dopamine from brain vesicular preparations: effects of adenosine analogues.

Authors:  R P Ebstein; J W Daly
Journal:  Cell Mol Neurobiol       Date:  1982-09       Impact factor: 5.046

6.  Adenosine receptor binding: structure-activity analysis generates extremely potent xanthine antagonists.

Authors:  R F Bruns; J W Daly; S H Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1983-04       Impact factor: 11.205

7.  Adenosine receptors and behavioral actions of methylxanthines.

Authors:  S H Snyder; J J Katims; Z Annau; R F Bruns; J W Daly
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205
  7 in total

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