Application of 31P-NMR saturation transfer techniques to investigate phospholipid motion and organization in model and biological membranes.

The potential of 31P-NMR saturation transfer experiments for determining motional characteristics (in the millisecond to second time scale) of phospholipids in model and biological membranes is demonstrated. A technique to separate membrane phospholipid 31P-NMR signals from those of small water-soluble phosphates in intact cells in liver tissue is also illustrated.