Literature DB >> 7412868

Free calcium in heart muscle at rest and during contraction measured with Ca2+ -sensitive microelectrodes.

E Marban, T J Rink, R W Tsien, R Y Tsien.   

Abstract

Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+ -sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 microM. During contractures, we recorded Ca2+ transients rising as high as 10 microM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.

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Year:  1980        PMID： 7412868     DOI： 10.1038/286845a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  76 in total

1.  Dependence of intracellular free calcium and tension on membrane potential and intracellular pH in single crayfish muscle fibres.

Authors:  K Kaila; J Voipio
Journal:  Pflugers Arch       Date:  1990-07       Impact factor: 3.657

2.  Heart failure and Ca++ activation of the cardiac contractile system: hereditary cardiomyopathy in hamsters (BIO 14.6), isoprenaline overload and the effect of APP 201-533.

Authors:  J W Herzig; W Gerber; R Salzmann
Journal:  Basic Res Cardiol       Date:  1987 Jul-Aug       Impact factor: 17.165

3.  Myofibrillar Ca++ activation and heart failure--Ca++ sensitization by the cardiotonic agent APP 201-533.

Authors:  J W Herzig; L H Botelho; R J Solaro
Journal:  Basic Res Cardiol       Date:  1989       Impact factor: 17.165

4.  Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice.

Authors:  Cecilia Giulivi; Catherine Ross-Inta; Alicja Omanska-Klusek; Eleonora Napoli; Danielle Sakaguchi; Genaro Barrientos; Paul D Allen; Isaac N Pessah
Journal:  J Biol Chem       Date:  2010-10-26       Impact factor: 5.157

5.  Periodic increase of cytoplasmic free calcium in fertilized hamster eggs measured with calcium-sensitive electrodes.

Authors:  Y Igusa; S Miyazaki
Journal:  J Physiol       Date:  1986-08       Impact factor: 5.182

6.  Sodium/calcium exchange and intracellular calcium buffering in ferret myocardium: an ion-sensitive micro-electrode study.

Authors:  R A Chapman
Journal:  J Physiol       Date:  1986-04       Impact factor: 5.182

7.  Intracellular free calcium concentration measured with 19F NMR spectroscopy in intact ferret hearts.

Authors:  E Marban; M Kitakaze; H Kusuoka; J K Porterfield; D T Yue; V P Chacko
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

8.  Degradation of skeletal muscle plasma membrane proteins by calpain.

Authors:  S I Zaidi; H T Narahara
Journal:  J Membr Biol       Date:  1989-09       Impact factor: 1.843

9.  Enhancement of calcium current during digitalis inotropy in mammalian heart: positive feed-back regulation by intracellular calcium?

Authors:  E Marban; R W Tsien
Journal:  J Physiol       Date:  1982-08       Impact factor: 5.182

10.  Protein kinase C enhances myosin light-chain kinase effects on force development and ATPase activity in rat single skinned cardiac cells.

Authors:  O Clement; M Puceat; M P Walsh; G Vassort
Journal:  Biochem J       Date:  1992-07-01       Impact factor: 3.857
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