Absorption by the colon during prolonged infusions in conscious, unrestrained rats.

1. Colonic absorption was studied in conscious, unrestrained rats during prolonged infusions through implanted cannulae. During infusion of predominantly NaCl-containing solution at rates up to 0.7 ml h-1 per 100 g body wt., absorption of fluid and Na increased considerably without significant change of transmucosal p.d.; diarrhoea did not occur. 2. Exclusion of the distal colon by colostomy showed that the proximal part of the colon was chiefly responsible for the increased absorption and inspection during the infusion showed it to be much dilated. Removal of the caecum showed that it contributed considerably to absorption by the proximal part. The distal colon influenced Na concentration in the faeces but absorbed little volume while direct infusion into this region rapidly produced diarrhoea. 3. The addition of Na deoxycholate (5 mmol/l) to the infusion solution impaired absorption provoking diarrhoea with mucus loss; there was no evidence that fluid secretion was stimulated. Substitution of SO4 for Cl in the infused solution produced only small changes of transmucosal p.d. but considerably impaired absorption and diarrhoea resulted. 4. The findings indicate that the proximal colon and caecum possess a considerable potential for increasing fluid and Na absorption and suggest the possibility that a neutral Na-Cl coupled absorptive mechanism is stimulated by loading the colon with fluid and NaCl.