Thyroid control over biomembranes. Liver-microsomal cytochrome b5 in hypothyroidism.

Hypothyroid rats were prepared by thyroidectomy and maintenance on a low-iodine diet (group A); Group B was additionally pretreated with 0.5 mCi of 131I as NaI, given intraperitoneally. Liver microsomes obtained from hypothyroid and normal rats were compared. After fasting and refeeding on 20% sucrose solution, high levels of microsomal fatty-acyl-CoA delta 9-desaturase (as measured spectrophotometrically by the rate constants for cytochrome b5 reoxidation) were induced in all the normal animals, half of the group A hypothyroid rats, and none of the group B hypothyroid rats. Hypothyroidism did not change desaturase Arrhenius profiles or V and Km for NADH-cytochrome c reductase, but increased content of cytochrome b5. The inability of adequately hypothyroid rats to induce the delta 9-desaturase seems to be specific, in that injection of methylcholanthrene successfully induced microsomal benzpyrene monooxygenase activity and increased cytochrome b5 contents in hypothyroid animals. The defects in overall fatty acyl desaturation reported in hypothyroid animals [Landriscina, C., Gnoni, G. V. & Quagliariello, E. (1976) Eur. J. Biochem. 71, 135-143] are suggested to be due to deficiencies in the specific desaturase(s).