Literature DB >> 7408853

Three-step purification of glucocorticoid receptors from rat liver.

M V Govindan, B Manz.   

Abstract

The 90 000-Mr glucocorticoid receptor was purified to homogeneity according to sodium dodecylsulfate/polyacrylamide gel electrophoresis. An affinity column containing either dexamethasone-17 beta-carboxylic acid or dexamethasone-21-methanesulfonate bound to the matrix with the help of a disulfide bond is used in this study. Using this affinity matrix, in a single step, 8700-fold purification of glucocorticoid receptor from rat liver cytosol could be achieved. Following the method of activation and DNA-cellulose chromatography the 90 000-Mr receptor subunit was purified to homogeneity.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7408853     DOI： 10.1111/j.1432-1033.1980.tb04694.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  8 in total

1.  An orthogonal dexamethasone-trimethoprim yeast three-hybrid system.

Authors:  Sarah S Gallagher; Lawrence W Miller; Virginia W Cornish
Journal:  Anal Biochem       Date:  2006-12-28       Impact factor: 3.365

2.  Investigation of the relative cellular permeability of DNA-binding pyrrole-imidazole polyamides.

Authors:  Bo Liu; Thomas Kodadek
Journal:  J Med Chem       Date:  2009-08-13       Impact factor: 7.446

3.  Immunochemical analysis of the glucocorticoid receptor: identification of a third domain separate from the steroid-binding and DNA-binding domains.

Authors:  J Carlstedt-Duke; S Okret; O Wrange; J A Gustafsson
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

4.  Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo.

Authors:  F Payvar; O Wrange; J Carlstedt-Duke; S Okret; J A Gustafsson; K R Yamamoto
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

5.  Purified glucocorticoid receptor-hormone complex from rat liver cytosol binds specifically to cloned mouse mammary tumor virus long terminal repeats in vitro.

Authors:  M V Govindan; E Spiess; J Majors
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

6.  Cloning of the human glucocorticoid receptor cDNA.

Authors:  M V Govindan; M Devic; S Green; H Gronemeyer; P Chambon
Journal:  Nucleic Acids Res       Date:  1985-12-09       Impact factor: 16.971

Review 7.  Glucocorticoid receptors in human leukemias and related diseases.

Authors:  E B Thompson; J R Smith; S Bourgeois; J M Harmon
Journal:  Klin Wochenschr       Date:  1985-08-01

8.  DNA binding properties of glucocorticosteroid receptors bound to the steroid antagonist RU-486.

Authors:  S Bourgeois; M Pfahl; E E Baulieu
Journal:  EMBO J       Date:  1984-04       Impact factor: 11.598
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.