Separate neurons in the paraventricular nucleus project to the median eminence and to the medulla or spinal cord.

Double labeling experiments with the retrogradely transported fluorescent tracers bisbenzimide and True blue indicate that separate populations of cells in the paraventricular nucleus of the hypothalamus project to the median eminence and to the dorsomedial medulla or spinal cord in the rat. The cell populations giving rise to each projection are, however, at least partially intermixed in different parts of the nucleus.