Literature DB >> 739986

Separation of nucleosomes containing histones H1 and H5.

T G Bakayeva, V V Bakayev.   

Abstract

Hen erythrocyte chromatin was digested with staphylococcal nuclease and fractionated by electrophoresis in polyacrylamide gels. Instead of the three bands described for mouse carcinoma chromatin, four main discrete components (MN1, MN2, MN2E and MN3) were resolved in the mononucleosome fraction of erythrocyte chromatin. MN2 contained all five histones and a DNA fragment of 165--180 base pairs. MN2E comprised four nucleosomal histones plus histone H5 (but not H1) and a DNA fragment of 170--190 base pairs. The relatively nuclease resistant MN3 fraction of erythrocyte nucleosomes contained H1 but no H5 histone. A more accurate analysis of the MN2 fraction in mouse carcinoma nucleosomes revealed some additional microheterogeneity depending on the presence of two different subfractions of H1.

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Year:  1978        PMID： 739986     DOI： 10.1007/bf00777522

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  21 in total

1.  Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

Authors:  P Oudet; M Gross-Bellard; P Chambon
Journal:  Cell       Date:  1975-04       Impact factor: 41.582

2.  Heterogeneity of chromatin subunits in vitro and location of histone H1.

Authors:  A J Varshavsky; V V Bakayev; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971

3.  Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.

Authors:  J P Whitlock; R T Simpson
Journal:  Biochemistry       Date:  1976-07-27       Impact factor: 3.162

4.  Nucleosomes and subnucleosomes: heterogeneity and composition.

Authors:  V V Bakayev; T G Bakayeva; A J Varshavsky
Journal:  Cell       Date:  1977-07       Impact factor: 41.582

5.  Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.

Authors:  D Lohr; J Corden; K Tatchell; R T Kovacic; K E Van Holde
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205

6.  Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA.

Authors:  Y V Ilyin; A Y Varshavsky; U N Mickelsaar; G P Georgiev
Journal:  Eur J Biochem       Date:  1971-09-24

7.  A study of the multiplicity of lysine-rich histones.

Authors:  M Bustin; R D Cole
Journal:  J Biol Chem       Date:  1969-10-10       Impact factor: 5.157

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  The specificity of histones in chicken erythrocytes.

Authors:  L S Hnilica
Journal:  Experientia       Date:  1964-01-15

10.  A comparison of the structure of chicken erythrocyte and chicken liver chromatin.

Authors:  N R Morris
Journal:  Cell       Date:  1976-12       Impact factor: 41.582
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  6 in total

1.  Histone H1o: its location in chromatin.

Authors:  B J Smith; E W Johns
Journal:  Nucleic Acids Res       Date:  1980-12-20       Impact factor: 16.971

2.  DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5.

Authors:  B L Miki; J M Neelin
Journal:  Nucleic Acids Res       Date:  1980-02-11       Impact factor: 16.971

3.  Nucleosome repeat lengths do not change during in vitro differentiation of erythroleukemia cells.

Authors:  R A Schlegel; A H Litwack; B M Phelps
Journal:  Mol Biol Rep       Date:  1980-07-31       Impact factor: 2.316

4.  Studies on protein organization of nucleosomes using cross-linking.

Authors:  V V Bakayev; T G Bakayeva; N N Domansky
Journal:  Mol Biol Rep       Date:  1981-08-14       Impact factor: 2.316

5.  Protection of discrete DNA fragments by the complex H1-octamerhistones or H5-octamerhistones after micrococcal nuclease digestion.

Authors:  S Muyldermans; I Lasters; L Wyns; R Hamers
Journal:  Nucleic Acids Res       Date:  1981-08-11       Impact factor: 16.971

6.  The organization of oligonucleosomes in yeast.

Authors:  C Szent-Gyorgyi; I Isenberg
Journal:  Nucleic Acids Res       Date:  1983-06-11       Impact factor: 16.971
  6 in total

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