Literature DB >> 7399543

Rat lens epithelial cells in vitro. II. Changes of protein patterns during aging and transformation.

H Rink, R Vornhagen.   

Abstract

Serially subcultured rat lens epithelial cells grow in different stages, which can be classified according to morphology, chromosome numbers and population kinetics. A lens-specific gamma-crystallin appears in the diploid stage, when elongated cell types are observed. One of the beta-crystallin bands (pH 5.7) disappears during aging in higher passage numbers of the diploid stage B. A weak band in the beta-crystallin region (pH 6.4), which is present in all stages, becomes very intensive in aneuploid cells of stage D, which exhibit a fibroblast-like morphology.

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Year:  1980        PMID： 7399543     DOI： 10.1007/bf02618332

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  12 in total

1.  ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS.

Authors:  C B LAURELL
Journal:  Anal Biochem       Date:  1965-02       Impact factor: 3.365

2.  Isoelectric focusing of embryonic cow lens crystallins.

Authors:  J Bours; S K Brahma
Journal:  Exp Eye Res       Date:  1973-06       Impact factor: 3.467

3.  Cultured calf lens epithelium. I. Methods of cultivation and characteristics of the cultures.

Authors:  W J van Venrooij; A A Groeneveld; H Bloemendal; E L Benedetti
Journal:  Exp Eye Res       Date:  1974-06       Impact factor: 3.467

4.  Molecular aspects of lens cell differentiation.

Authors:  J Papaconstantinou
Journal:  Science       Date:  1967-04-21       Impact factor: 47.728

5.  Isoelectric focusing of lens crystallins in thin-layer polyacrylamide gels. A method for detection of soluble proteins in eye lens extracts.

Authors:  J Bours
Journal:  J Chromatogr       Date:  1971-08-26

6.  Characteristics of cultured lens epithelium.

Authors:  A L Shapiro; I M Siegel; M D Scharff; E Robbins
Journal:  Invest Ophthalmol       Date:  1969-08

7.  Rat lens epithelial cells in vitro. I. Observations on aging, differentiation and culture alterations.

Authors:  H Rink; R Vornhagen; H R Koch
Journal:  In Vitro       Date:  1980-01

8.  Differentiation of rat lens epithelial cells in tissue culture. (I) Effects of cell density, medium and embryonic age of initial culture.

Authors:  M O Creighton; G Y Mousa; J R Trevithick
Journal:  Differentiation       Date:  1976-10-07       Impact factor: 3.880

9.  Tissue culture of lens epithelial cells from normal and Nakano mice.

Authors:  P Russell; H N Fukui; Y Tsunematsu; F L Huang; J H Konoshita
Journal:  Invest Ophthalmol Vis Sci       Date:  1977-03       Impact factor: 4.799

10.  Cell elongation in the cultured embryonic chick lens epithelium with and without protein synthesis. Involvement of microtubules.

Authors:  J Piatigorsky; H de F Webster; M Wollberg
Journal:  J Cell Biol       Date:  1972-10       Impact factor: 10.539
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