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Literature DB >> 7396861

Isolation and characterization of riboflavin-binding protein from pregnant-rat serum.

Abstract

A high-affinity riboflavin -binding protein was isolated and characterized for the first time from pregnant-rat sera by affinity chromatography on a lumiflavin-agarose column. The purified protein was homogeneous by the criteria of analytical polyacrylamide-gel disc electrophoresis, gel-filtration chromatography on Sephadex G-100 and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. It had a molecular weight of 90000+/-5000 and interacted with [14C]riboflavin with a 1:1 molar ratio with a dissociation constant (Kd) of 0.42 micron.

Entities: Chemical Species

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Year: 1980 PMID： 7396861 PMCID： PMC1161823 DOI： 10.1042/bj1870537

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

1. The interaction of riboflavin with a protein isolated from hen's egg white: a spectrofluorimetric study.

Authors: U S Murthy; S K Podder; P R Adiga
Journal: Biochim Biophys Acta Date: 1976-05-20

2. Synthesis of immobilized flavin derivatives and their use in purification of chicken egg-white ovoflavoprotein.

Authors: G Blankenhorn; D T Osuga; H S Lee; R E Feeney
Journal: Biochim Biophys Acta Date: 1975-04-29

3. Avian riboflavinuria. 8. The fate of the riboflavin-binding protein-riboflavin complex during incubation of hen's eggs.

Authors: C H Hammer; E G Buss; C O Clagett
Journal: Poult Sci Date: 1973-03 Impact factor: 3.352

1. Enzymic basis of deranged foetal flavin-nucleotide metabolism consequent on immunoneutralization of maternal riboflavin carrier protein in the pregnant rat.

Authors: N Surolia; K Krishnamurthy; P R Adiga
Journal: Biochem J Date: 1985-09-01 Impact factor: 3.857

1 in total

Isolation and characterization of riboflavin-binding protein from pregnant-rat serum.

1. The interaction of riboflavin with a protein isolated from hen's egg white: a spectrofluorimetric study.

2. Synthesis of immobilized flavin derivatives and their use in purification of chicken egg-white ovoflavoprotein.

3. Avian riboflavinuria. 8. The fate of the riboflavin-binding protein-riboflavin complex during incubation of hen's eggs.

4. Plasma protein binding of riboflavin and riboflavin-5'-phosphate in man.

5. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

6. Recovery of S35 radioactivity from protein-bearing polyacrylamide gel.

7. Riboflavin-binding protein of hen's egg : purification & radioimmunoassay.

8. Oestrogen induction of riboflavin-binding protein in immature chicks.

9. Distribution of riboflavin in blood: in women and in prenates.

10. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

1. Enzymic basis of deranged foetal flavin-nucleotide metabolism consequent on immunoneutralization of maternal riboflavin carrier protein in the pregnant rat.