Literature DB >> 7391591

Calculation of average antibody affinity in anti-hapten sera from data obtained by competitive radioimmunoassay.

R Müller.   

Abstract

A method is described for rapid and precise determination of average antibody affinity in anti-hapten sera from the following data obtained by competitive radioimmunoassay (RIA): the molar hapten (inhibitor) concentration giving 50% inhibition of tracer-antibody binding under equilibrium conditions, the molar tracer concentration in the assay and the amount of tracer bound by the antibodies in the absence of inhibitor. Thus, no additional experiments are required once a competitive RIA has been established. As an example, the average antibody affinity constants for 2'-deoxyguanosine, O6-ethyl-guanosine, and O6-ethyl-2'-deoxyguanosine were determined in antisera raised against the respective nucleoside-protein conjugates.

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Year:  1980        PMID： 7391591     DOI： 10.1016/0022-1759(80)90107-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  19 in total

1.  Production and characterization of 22 monoclonal antibodies directed against S 20499, a new potent 5-HT1A chiral agonist: influence of the hapten structure on specificity and stereorecognition.

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Journal:  Pharm Res       Date:  1999-05       Impact factor: 4.200

2.  Characterization of the immunochemical reactivity of fibrinogen fragments by competitive radioimmunoassay: an improved method of analysis.

Authors:  D J Winzor; J A Nagy; H A Scheraga
Journal:  J Protein Chem       Date:  1991-12

3.  Characterization of murine monoclonal antibodies that recognize neutralizing epitopes on human C5a.

Authors:  J W Larrick; J Wang; B M Fendly; D E Chenoweth; S L Kunkel; T Deinhart
Journal:  Infect Immun       Date:  1987-08       Impact factor: 3.441

Review 4.  Antibodies specific for DNA components structurally modified by chemical carcinogens.

Authors:  R Müller; M F Rajewsky
Journal:  J Cancer Res Clin Oncol       Date:  1981       Impact factor: 4.553

5.  Development of aflatoxin B(1)-lysine adduct monoclonal antibody for human exposure studies.

Authors:  J S Wang; S Abubaker; X He; G Sun; P T Strickland; J D Groopman
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

6.  Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column.

Authors:  E M Park; M K Shigenaga; P Degan; T S Korn; J W Kitzler; C M Wehr; P Kolachana; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

7.  Tissue-penetrating delivery of compounds and nanoparticles into tumors.

Authors:  Kazuki N Sugahara; Tambet Teesalu; Priya Prakash Karmali; Venkata Ramana Kotamraju; Lilach Agemy; Olivier M Girard; Douglas Hanahan; Robert F Mattrey; Erkki Ruoslahti
Journal:  Cancer Cell       Date:  2009-12-08       Impact factor: 31.743

8.  A bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine x bovine) xbovine interspecies fusion.

Authors:  D J Groves; J Clayton; B A Morris
Journal:  Vet Immunol Immunopathol       Date:  1988-02       Impact factor: 2.046

9.  Development of a sensitive radioimmunoassay for Fab fragments: application to Fab pharmacokinetics in humans.

Authors:  C V Thanh-Barthet; M Urtizberea; A E Sabouraud; N J Cano; J M Scherrmann
Journal:  Pharm Res       Date:  1993-05       Impact factor: 4.200

10.  High-affinity monoclonal antibodies for aflatoxins and their application to solid-phase immunoassays.

Authors:  J D Groopman; L J Trudel; P R Donahue; A Marshak-Rothstein; G N Wogan
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205
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