Inducible effect of colchicine on alkaline phosphatase in rat liver in vivo.

A single injection of colchicine, 1 mg/kg body weight, remarkably increased the activity of alkaline phosphatase in rat livers, followed by its increase in serum. Combination of bile duct ligation with colchicine administration had an additional effect on the alkaline phosphatase level above those induced by the individual treatments alone. Treatment with cycloheximide significantly inhibited the inducible effect of colchicine on the enzyme suggesting that protein synthesis is prerequisite to the elevation of the alkaline phosphatase caused by colchicine treatment.