Effect of chemotherapeutic agents on the formation of estrogen-receptor complex in human breast tumor cytosol.

We have examined the effect of Adriamycin (doxorubicin), cytoxan, methotrexate, and oncovin on [3H]estradiol binding to the cytoplasmic estrogen receptor of rat uterus and human breast tumors. When the estrogen binding assay was performed in the presence of these drugs over concentrations ranging from 1 x 10(-8) to 1 x 10(-4) M, no effect on the formation of specific [3H]estradiol-receptor complexes as determined by the dextran-coated charcoal method was observed.