| Literature DB >> 7381338 |
M L Kashyap, B A Hynd, K Robinson.
Abstract
When total protein in very low density lipoprotein samples is measured by the method of Lowry et al. (1951. J. Biol. Chem 193:265-275), turbidity remains in the final color reaction. Addition of 0.1 ml of 2.5% (v/v) solution of Triton X-100 removes this turbidity immediately and effectively. This simple modification is faster, less cumbersome, and more economical than removing turbidity with ethyl ether or chloroform. Addition of 0.1 ml of 2.5% (w/v) sodium dodecyl sulfate to the final reaction mixture is also effective in removing turbidity and can be used as an alternative method.Entities:
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Year: 1980 PMID： 7381338
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922