Literature DB >> 7381009

Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay.

B Adler, A M Murphy, S A Locarnini, S Faine.   

Abstract

The enzyme-linked immunosorbent assay (ELISA) was used to detect leptospire-specific immunoglobulin M (IgM) and IgG in the sera of patients infected with leptospiral serovars hardjo, pomona, or copenhageni. All patients produced specific IgM and IgG detectable by ELISA. In contrast, only a few patients produced IgG agglutinins whereas all produced IgM agglutinins. The specificity and sensitivity of the test suggest that the ELISA anti-IgM technique is a suitable method for detecting leptospiral antibodies in human sera for diagnostic and epidemiological purposes.

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Year:  1980        PMID： 7381009      PMCID： PMC273430          DOI： 10.1128/jcm.11.5.452-457.1980

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

2.  Immunological response in leptospirosis. Report of three cases.

Authors:  M J Tong; E B Rosenberg; B A Votteri; C C Tsai
Journal:  Am J Trop Med Hyg       Date:  1971-07       Impact factor: 2.345

3.  The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.

Authors:  S Avrameas; T Ternynck
Journal:  Immunochemistry       Date:  1969-01

4.  Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis.

Authors:  J A Morris; S N Hussaini
Journal:  J Hyg (Lond)       Date:  1974-12

5.  Molecular characteristics of antibody detected by the microscopic agglutination test in serum of guinea pigs with leptospirosis.

Authors:  R P Crawford
Journal:  Am J Vet Res       Date:  1972-07       Impact factor: 1.156

6.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors:  E Engvall; P Perlmann
Journal:  Immunochemistry       Date:  1971-09

Review 7.  Leptospirosis. II. Serology.

Authors:  L H Turner
Journal:  Trans R Soc Trop Med Hyg       Date:  1968       Impact factor: 2.184

8.  Chromatographic analysis and sulfhydryl sensitivity of antileptospira agglutinins in rabbit and human sera.

Authors:  R M Pike; H L McBrayer; M L Schulze; C H Chandler
Journal:  Proc Soc Exp Biol Med       Date:  1965-12

9.  Microtiter solid-phase radioimmunoassay for hepatitis B antigen.

Authors:  R H Purcell; D C Wong; H J Alter; P V Holland
Journal:  Appl Microbiol       Date:  1973-10

10.  Antileptospiral agglutinins produced in rabbits.

Authors:  S Graves; S Faine
Journal:  Bull World Health Organ       Date:  1970       Impact factor: 9.408
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  44 in total

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Authors:  H L Smits; C K Eapen; S Sugathan; M Kuriakose; M H Gasem; C Yersin; D Sasaki; B Pujianto; M Vestering; T H Abdoel; G C Gussenhoven
Journal:  Clin Diagn Lab Immunol       Date:  2001-01

Review 2.  Leptospirosis.

Authors:  P N Levett
Journal:  Clin Microbiol Rev       Date:  2001-04       Impact factor: 26.132

3.  An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK.

Authors:  W J Zochowski; M F Palmer; T J Coleman
Journal:  J Clin Pathol       Date:  2001-01       Impact factor: 3.411

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Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

5.  Seroepidemiology of leptospirosis in livestock in Trinidad.

Authors:  Sharianne M Suepaul; Christine V Carrington; Mervyn Campbell; Gustave Borde; Abiodun Adewale Adesiyun
Journal:  Trop Anim Health Prod       Date:  2010-10-16       Impact factor: 1.559

6.  Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.

Authors:  H Guerreiro; J Croda; B Flannery; M Mazel; J Matsunaga; M Galvão Reis; P N Levett; A I Ko; D A Haake
Journal:  Infect Immun       Date:  2001-08       Impact factor: 3.441

7.  Development and initial evaluation of an indirect enzyme-linked immunosorbent assay for the detection of Leptospira interrogans serovar hardjo antibodies in bovine sera.

Authors:  O P Surujballi; R M Marenger; M D Eaglesome; E A Sugden
Journal:  Can J Vet Res       Date:  1997-10       Impact factor: 1.310

8.  Development of a monoclonal antibody-based competitive enzyme-linked immunosorbent assay for the detection of Leptospira borgpetersenii serovar hardjo type hardjobovis antibodies in bovine sera.

Authors:  O Surujballi; D Henning; R Marenger; C Howlett
Journal:  Can J Vet Res       Date:  1997-10       Impact factor: 1.310

9.  Evaluation of a commercial latex agglutination assay for serological diagnosis of leptospirosis.

Authors:  C Hull-Jackson; M B Glass; M D Ari; S L Bragg; S L Branch; C U Whittington; C N Edwards; P N Levett
Journal:  J Clin Microbiol       Date:  2006-05       Impact factor: 5.948

10.  New approach for serological testing for leptospirosis by using detection of leptospira agglutination by flow cytometry light scatter analysis.

Authors:  S Yitzhaki; A Barnea; A Keysary; E Zahavy
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948
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