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Literature DB >> 7372571

Deoxyribonucleic acid polymerase from the marine Pseudomonas sp. BAL-31.

R Vicuña, F Valdés, A Medina, A Yudelevich.

Abstract

A deoxyribonucleic acid (DNA)-dependent DNA polymerase (DNA nucleotidyltransferase) was purified 3,000-fold from the marine Pseuodomonas sp. BAL-31. The molecular weight of the native enzyme was estimated by glycerol gradient sedimentation to be 110,000. The enzyme migrated in sodium dodecyl sulfate-acrylamide gels as a single polypeptide with a molecular weight of 105,000. An absolute requirement for divalent cation was satisfied by Mg2+ or Mn2+ at concentrations of 1 mM. Monovalent cations at concentrations higher than 50 mM showed an inhibitory effect. The polymerase activity was resistant to N-ethylmaleimide and showed a wide pH optimum.

Entities: Chemical Species

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Year: 1980 PMID： 7372571 PMCID： PMC293940 DOI： 10.1128/jb.142.1.249-253.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

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References

25 in total

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Authors: W Wickner; R Schekman; K Geider; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1973-06 Impact factor: 11.205

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Authors: A Kornberg
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Authors: A T Ganesan; C O Yehle; C C Yu
Journal: Biochem Biophys Res Commun Date: 1973-01-04 Impact factor: 3.575

Deoxyribonucleic acid polymerase from the marine Pseudomonas sp. BAL-31.

1. A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template.

2. Active center of DNA polymerase.

3. DNA replication in a polymerase I deficient mutant and the identification of DNA polymerases II and 3 in Bacillus subtilis.

4. Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.

5. Properties of bacteriophage PM2: a lipid-containing bacterial virus.

6. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

7. Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases.

8. Purification and properties of deoxyribonucleic acid polymerase from Micrococcus lysodeikticus.

9. Ultrastructure of bacterial cells infected with bacteriophage PM2, a lipid-containing bacterial virus.

10. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli.