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Literature DB >> 7372341

The identification of human chromosomes by quinacrine fluorescence after hybridisation in situ.

Abstract

A method is described for producing fluorescent bands on human chromosomes by staining with quinacrine after hybridisation in situ. The advantages of the method include the eslimination of artefacts arising from staining before hybridisation, the fact that there is no reduction in sample number between staining and autoradiography, the ease with which autoradiographic grains can be identified and counted. and the reduction in exposure time.

Entities: Chemical Species

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Year: 1980 PMID： 7372341 DOI： 10.1007/bf00287058

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

12 in total

1. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes.

Authors: M E Chandler; J J Yunis
Journal: Cytogenet Cell Genet Date: 1978

2. A simple procedure for obtaining autoradiographs of G-banded chromosomes.

Authors: M H Rutledge
Journal: Chromosoma Date: 1979-01-08 Impact factor: 4.316

3. Satellite DNA loss and nucleolar organiser activity in an individual with a de novo chromosome 13,14 translocation.

Authors: J R Gosden; C M Gosden; S S Lawrie; K E Buckton
Journal: Clin Genet Date: 1979-06 Impact factor: 4.438

4. Mechanisms involved in the banding of chromosomes with guinacrine and Giems. II. The interaction of the dyes with the chromosomal components.

Authors: A T Sumner; H J Evans
Journal: Exp Cell Res Date: 1973-09 Impact factor: 3.905

5. Cytological mapping of human chromosomes: results obtained with quinacrine fluorescence and the acetic-saline-Giemsa techniques.

Authors: H J Evans; K E Buckton; A T Sumner
Journal: Chromosoma Date: 1971 Impact factor: 4.316

The identification of human chromosomes by quinacrine fluorescence after hybridisation in situ.

1. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes.

2. A simple procedure for obtaining autoradiographs of G-banded chromosomes.

3. Satellite DNA loss and nucleolar organiser activity in an individual with a de novo chromosome 13,14 translocation.

4. Mechanisms involved in the banding of chromosomes with guinacrine and Giems. II. The interaction of the dyes with the chromosomal components.

5. Cytological mapping of human chromosomes: results obtained with quinacrine fluorescence and the acetic-saline-Giemsa techniques.

6. Simultaneous observation of quinacrine bands and silver grains on radiolabeled metaphase chromosomes.

7. An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes.

8. Quinacrine fluorescence and Q-banding patterns of human chromosomes. I. Effects of varying factors.

9. The location of four human satellite DNAs on human chromosomes.

10. Suppression of quinacrine banding of human chromosomes by mounting in organic media.

1. Satellite DNA sequences in the human acrocentric chromosomes: information from translocations and heteromorphisms.

2. Satellite DNA and cytological staining patterns in heterochromatic inversions of human chromosome 9.

3. Familial reciprocal translocation t(9;13)(p11;p12) investigated by silver staining and in situ hybridisation.

4. Localization of actin-related sequences by in situ hybridization to R-banded human chromosomes.

5. Characterisation of a human Y chromosome repeated sequence and related sequences in higher primates.

6. Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.