Characterization of a novel endo-N-acetyl-beta-D-glucosaminidase from the culture filtrate of a basidiomycete (Sporotricum dimorphosporum), active on biantennary mono- and asialoglycoasparagines of the N-acetyllactosaminic type.

A novel endo-N-acetyl-beta-D-glucosaminidase has been characterized in a culture filtrate from a Basidiomycete. This enzyme hydrolyses biantennary monosialo and asialo-glycoasparagines of the N-acetyllactosaminic type. Endo-N-acetyl-beta-D-glucosaminidase from the Basidiomycete released from different glycoasparagines, beta-GlcNAc-(1 leads to 4)-N[14C] acetyl-Asn, alpha Fuc-(1 leads to 6)-beta-GlcNAc-(1 leads to 4)-N-[14C]acetyl-Asn and oligosaccharides which have been separated by paper and thin-layer chromatography. Determination of the radioactivity of the labeled fragments leads to the conclusion that the biantennary glycoasparagines of the N-acetyllactosaminic type are hydrolyzed after 1 h incubation at 37 degrees, to the extent of 15 per cent for the monosialo; 90 per cent for the asialo; 21 per cent for the asialo-monofucosylated, and 15 per cent for the asialo-difucosylated glycans. Tri- and tetraantennary asialo-glycans of the N-acetyllactosaminic type are not hydrolyzed by this enzyme.