| Literature DB >> 7358995 |
L S Simon, R Patterson, T L Jones.
Abstract
A new rapid method for assessing murine macrophage chemotaxis was developed using peritoneal exudate cells labeled with [5,6-3H]uridine in modified Boyden chambers with double polycarbonate filters. The method gave positive results with endotoxin-treated mouse serum and with serum-free culture supernatant of the DBA/2 SaD2 fibrosarcoma. These results correlated with results obtained with conventional microscopic assessment of chemotaxis. Using this method, the SaD2 supernatant was shown to be chemotactic rather than chemokinetic.Entities:
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Year: 1980 PMID： 7358995 DOI： 10.1016/0022-1759(80)90073-3
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303