Literature DB >> 7356746

A rapid and reproducible method for the separation of cells from radioactive media.

A I Kassis, S J Adelstein.   

Abstract

A simple method is described for the rapid separation of cells, containing radioactive material, from their suspending medium. Cells, in aqueous medium, are layered on fetal bovine serum in microfuge tubes. The tubes are immediately spun for 1 min, frozen in ethanol-dry-ice, and their tips sliced and assayed. This method is both faster and more convenient than currently employed techniques.

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Year:  1980        PMID： 7356746

Source DB:  PubMed          Journal:  J Nucl Med        ISSN： 0161-5505            Impact factor:   10.057


  5 in total

1.  Evidence for pronounced bystander effects caused by nonuniform distributions of radioactivity using a novel three-dimensional tissue culture model.

Authors:  A Bishayee; D V Rao; R W Howell
Journal:  Radiat Res       Date:  1999-07       Impact factor: 2.841

Review 2.  Auger electron emitters: insights gained from in vitro experiments.

Authors:  G Makrigiorgos; S J Adelstein; A I Kassis
Journal:  Radiat Environ Biophys       Date:  1990       Impact factor: 1.925

3.  Biological effect of lead-212 localized in the nucleus of mammalian cells: role of recoil energy in the radiotoxicity of internal alpha-particle emitters.

Authors:  M T Azure; R D Archer; K S Sastry; D V Rao; R W Howell
Journal:  Radiat Res       Date:  1994-11       Impact factor: 2.841

4.  Radioprotection against lethal damage caused by chronic irradiation with radionuclides in vitro.

Authors:  R W Howell; S M Goddu; A Bishayee; D V Rao
Journal:  Radiat Res       Date:  1998-10       Impact factor: 2.841

5.  Radiotoxicity of platinum-195m-labeled trans-platinum (II) in mammalian cells.

Authors:  R W Howell; A I Kassis; S J Adelstein; D V Rao; H A Wright; R N Hamm; J E Turner; K S Sastry
Journal:  Radiat Res       Date:  1994-10       Impact factor: 3.372
  5 in total

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