Literature DB >> 7356711

A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA.

D Eilat, R Asofsky, R Laskov.   

Abstract

Spleen cells from an unimmunized autoimmune NZB/NZW F1 female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (10(9) liter/mole) for the RNA antigen.

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Year:  1980        PMID： 7356711

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  11 in total

Review 1.  B-cell epitopes of RNA autoantigens.

Authors:  R M Hoet; W J van Venrooij
Journal:  Mol Biol Rep       Date:  1992-06       Impact factor: 2.316

Review 2.  Hybridomas: a new dimension in biological analyses.

Authors:  R H Kennett
Journal:  In Vitro       Date:  1981-12

3.  Specific Recognition of a Single-Stranded RNA Sequence by a Synthetic Antibody Fragment.

Authors:  Yaming Shao; Hao Huang; Daoming Qin; Nan-Sheng Li; Akiko Koide; Jonathan P Staley; Shohei Koide; Anthony A Kossiakoff; Joseph A Piccirilli
Journal:  J Mol Biol       Date:  2016-09-02       Impact factor: 5.469

4.  Monoclonal antibodies showing sequence specificity in their interaction with single-stranded DNAs.

Authors:  J S Lee; J R Lewis; A R Morgan; T R Mosmann; B Singh
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971

5.  Production of autoantibodies by human-human hybridomas.

Authors:  Y Shoenfeld; S C Hsu-Lin; J E Gabriels; L E Silberstein; B C Furie; B Furie; B D Stollar; R S Schwartz
Journal:  J Clin Invest       Date:  1982-07       Impact factor: 14.808

6.  Antibodies to RNA from autoimmune NZB/NZW mice recognize a similar antigenic determinant and show a large idiotypic diversity.

Authors:  D Eilat; M Hochberg; R Fischel; R Laskov
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

7.  Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype.

Authors:  P M Atkinson; G W Lampman; B C Furie; Y Naparstek; R S Schwartz; B D Stollar; B Furie
Journal:  J Clin Invest       Date:  1985-04       Impact factor: 14.808

8.  Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

Authors:  E A Lerner; M R Lerner; C A Janeway; J A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

9.  Use of monoclonal antibodies for the characterization of novel DNA-binding proteins recognized by human autoimmune sera.

Authors:  W H Reeves
Journal:  J Exp Med       Date:  1985-01-01       Impact factor: 14.307

10.  Description and partial characterization of a nucleolar RNA-associated autoantigen defined by a human monoclonal antibody.

Authors:  N Chiorazzi; W H Reeves
Journal:  J Exp Med       Date:  1987-04-01       Impact factor: 14.307
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