Literature DB >> 7353311

A fluorometric assay for measurement of protoporphyrinogen oxidase activity in mammalian tissue.

D A Brenner, J R Bloomer.   

Abstract

The oxidation of protoporphyrinogen to protoporphyrin, which is catalyzed by the enzyme protoporphyrinogen oxidase, is an important step in heme biosynthesis. We describe a fluorometric assay for protoporphyrinogen oxidase activity in mammalian tissues which measures the conversion of the non-fluorescent protoporphyrinogen to the fluorescent protoporphyrin. Using these assay conditions, the mean level of protoporphyrinogen oxidase activity in sonicated rat liver mitochondria was 12.3, and in sonicated human cultured skin fibroblasts was 1.97 nmol protoporphyrin/mg protein/h.

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Year:  1980        PMID： 7353311     DOI： 10.1016/0009-8981(80)90275-2

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  13 in total

1.  Human protoporphyrinogen oxidase: expression, purification, and characterization of the cloned enzyme.

Authors:  T A Dailey; H A Dailey
Journal:  Protein Sci       Date:  1996-01       Impact factor: 6.725

2.  An h.p.l.c. assay for protoporphyrinogen oxidase activity in rat liver.

Authors:  F Li; C K Lim; T J Peters
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

3.  Porphyrin metabolism and haem biosynthesis in Gilbert's syndrome.

Authors:  K E McColl; G G Thompson; E el Omar; M R Moore; A Goldberg
Journal:  Gut       Date:  1987-02       Impact factor: 23.059

4.  Effect of rifampicin on haem and bilirubin metabolism in man.

Authors:  K E McColl; G G Thompson; E el Omar; M R Moore; B K Park; M J Brodie
Journal:  Br J Clin Pharmacol       Date:  1987-05       Impact factor: 4.335

5.  Inhibition of plant protoporphyrinogen oxidase by the herbicide acifluorfen-methyl.

Authors:  D A Witkowski; B P Halling
Journal:  Plant Physiol       Date:  1989-08       Impact factor: 8.340

6.  A continuous fluorimetric assay for protoporphyrinogen oxidase by monitoring porphyrin accumulation.

Authors:  Mark Shepherd; Harry A Dailey
Journal:  Anal Biochem       Date:  2005-09-01       Impact factor: 3.365

7.  Allosteric inhibition of human lymphoblast and purified porphobilinogen deaminase by protoporphyrinogen and coproporphyrinogen. A possible mechanism for the acute attack of variegate porphyria.

Authors:  P Meissner; P Adams; R Kirsch
Journal:  J Clin Invest       Date:  1993-04       Impact factor: 14.808

8.  The inherited enzymatic defect in porphyria variegata.

Authors:  J C Deybach; H de Verneuil; Y Nordmann
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

9.  Biphasic ordered induction of heme synthesis in differentiating murine erythroleukemia cells: role of erythroid 5-aminolevulinate synthase.

Authors:  H Lake-Bullock; H A Dailey
Journal:  Mol Cell Biol       Date:  1993-11       Impact factor: 4.272

10.  Mouse protoporphyrinogen oxidase. Kinetic parameters and demonstration of inhibition by bilirubin.

Authors:  G C Ferreira; H A Dailey
Journal:  Biochem J       Date:  1988-03-01       Impact factor: 3.857
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