Use of solid medium for isolation of leptospires of the Hebdomadis serogroup from bovine milk and urine.

Leptospira interrogans serovars hardjo and szwajizak were isolated from the milk of experimentally infected cows only when using bovine albumin polysorbate-80 solid medium. Semisolid media, with and without the addition of 5-fluorouracil, and hamster inoculations were also used in the isolation attempts. These cultural isolation methods were also compared using cows' urine. Semisolid medium with 5-fluorouracil proved to be the most successful. However, the other methods were satisfactory, with solid medium having the lowest percentage of cultural isolations.