Literature DB >> 7337709

A convenient zymogram stain for cellulases.

A McHale, M P Coughlan.   

Abstract

The release of dye from phosphoric acid-swollen cellulose azure by endocellulases and exocellulases is the basis of a technique for locating these enzymes on gels.

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Year:  1981        PMID： 7337709      PMCID： PMC1163362          DOI： 10.1042/bj1990267

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  The use of reactive dyestuffs in enzymology: new substrates for cellulolytic enzymes.

Authors:  H N FERNLEY
Journal:  Biochem J       Date:  1963-04       Impact factor: 3.857

2.  Measurement of saccharifying cellulase.

Authors:  M Mandels; R Andreotti; C Roche
Journal:  Biotechnol Bioeng Symp       Date:  1976

3.  Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors:  R A REISFELD; U J LEWIS; D E WILLIAMS
Journal:  Nature       Date:  1962-07-21       Impact factor: 49.962

4.  Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 1. Separation, purification and physico-chemical characterization of five endo-1,4-beta-glucanases.

Authors:  K E Eriksson; B Pettersson
Journal:  Eur J Biochem       Date:  1975-02-03

5.  Glycoprotein staining following electrophoresis on acrylamide gels.

Authors:  R M Zacharius; T E Zell; J H Morrison; J J Woodlock
Journal:  Anal Biochem       Date:  1969-07       Impact factor: 3.365

6.  A zymogram technique for the detection of carbohydrases.

Authors:  K E Eriksson; B Pettersson
Journal:  Anal Biochem       Date:  1973-12       Impact factor: 3.365

7.  Purification and properties of the cellulases from the thermophilic fungus Thermoascus aurantiacus.

Authors:  C C Tong; A L Cole; M G Shepherd
Journal:  Biochem J       Date:  1980-10-01       Impact factor: 3.857

8.  Synergistic hydrolysis of cellulose by components of the extracellular cellulase system of Talaromyces emersonii.

Authors:  A McHale; M P Coughlan
Journal:  FEBS Lett       Date:  1980-08-11       Impact factor: 4.124

9.  Immunoelectrophoretic detection of cellulases.

Authors:  M Nummi; M L Niku-Paavola; T M Enari; V Raunio
Journal:  FEBS Lett       Date:  1980-05-05       Impact factor: 4.124

10.  The cellulolytic enzymes of Botryodiplodia theobromae Pat. Separation and characterization of cellulases and beta-glucosidases.

Authors:  G M Umezurike
Journal:  Biochem J       Date:  1979-01-01       Impact factor: 3.857
  10 in total
  2 in total

1.  Isolation and characterization of the 1,4-beta-D-glucan glucanohydrolases of Talaromyces emersonii.

Authors:  A P Moloney; S I McCrae; T M Wood; M P Coughlan
Journal:  Biochem J       Date:  1985-01-15       Impact factor: 3.857

2.  From simple and specific zymographic detections to the annotation of a fungus Daldinia caldariorum D263 that encodes a wide range of highly bioactive cellulolytic enzymes.

Authors:  Meng-Chun Lin; Hsion-Wen Kuo; Mu-Rong Kao; Wen-Dar Lin; Chen-Wei Li; Kuo-Sheng Hung; Sheng-Chih Yang; Su-May Yu; Tuan-Hua David Ho
Journal:  Biotechnol Biofuels       Date:  2021-05-21       Impact factor: 6.040
  2 in total

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