Literature DB >> 7334214

A micromethod for the induction and assay of specific in vitro antibody responses by human lymphocytes.

E D Zanders, C M Smith, R E Callard.   

Abstract

Human lymphocytes obtained from peripheral blood or spleen were cultured with influenza virus in a microculture system to produce strain specific antibody which was detected by the enzyme linked immunosorbent assay (ELISA). Nanogram amounts of specific antibody could be detected in cultures of 4 x 10(5) cells.

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Year:  1981        PMID: 7334214     DOI: 10.1016/0022-1759(81)90289-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  9 in total

1.  Absence of monocyte procoagulant activity during the immune response to influenza virus.

Authors:  M Mathies; N Hogg
Journal:  J Clin Microbiol       Date:  1989-04       Impact factor: 5.948

2.  Induction of a T-cell mediated suppressor activity by soluble products from antigen-specific helper/inducer human T-cell lines.

Authors:  A Fischer; P Beverley; M Feldmann; S Smith
Journal:  Immunology       Date:  1983-01       Impact factor: 7.397

3.  Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines.

Authors:  E D Zanders; A Fischer; S Smith; P C Beverley; M Feldmann
Journal:  Immunology       Date:  1983-02       Impact factor: 7.397

4.  Regulation of antibody production by an antigen-specific EBV-transformed B-lymphoblastoid cell line: effect of high-dose antigen and antigen-pulsed T cells.

Authors:  G W McCaughan; M H Brown; R E Callard
Journal:  Immunology       Date:  1986-08       Impact factor: 7.397

5.  Relative sensitivity of human T cell subsets to deoxyadenosine toxicity.

Authors:  R E Callard; T M Ewing; R M Fox
Journal:  Clin Exp Immunol       Date:  1984-10       Impact factor: 4.330

6.  The dissociation of interleukin-2 production and antigen-specific helper activity by clonal analysis.

Authors:  J R Lamb; E D Zanders; M Feldmann; P Lake; D D Eckels; J N Woody; P C Beverley
Journal:  Immunology       Date:  1983-11       Impact factor: 7.397

7.  Specific antibody responses by high- and low-density human peripheral blood B cells: T-helper cells and T-cell replacing factor (TRF) act on different B-cell subpopulations.

Authors:  R E Callard; S L Tiernan
Journal:  Immunology       Date:  1987-11       Impact factor: 7.397

8.  Induction of tolerance in influenza virus-immune T lymphocyte clones with synthetic peptides of influenza hemagglutinin.

Authors:  J R Lamb; B J Skidmore; N Green; J M Chiller; M Feldmann
Journal:  J Exp Med       Date:  1983-05-01       Impact factor: 14.307

9.  Antigen-specific suppression of human antibody responses by allogeneic T cells. I. Frequency and antigen specificity of allogeneic suppressor T cells and their role in major histocompatibility complex-controlled genetic restriction.

Authors:  R E Callard; C M Winger; S L Tiernan
Journal:  J Exp Med       Date:  1984-04-01       Impact factor: 14.307

  9 in total

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