Genetics of the Akp-2 locus for alkaline phosphatase of liver, kidney, bone, and placenta in the mouse. Linkage with the Ahd-1 locus on chromosome 4.

Differences between strains of mice were observed in electrophoretic migration on cellulose acetate plates of alkaline phosphatase in butanol extracts of kidney, bone and placenta. Each strain exhibited a broad band with fast or slow migration. Distribution among strains including recombinant inbred (Rl) strains indicated the variation in these organs to be the same as the previously described in the liver as coded for by the Akp-2 locus. An analysis of Rl strains for the kidney isozyme showed 43 with the faster moving form (AKP-2A), and 43 with the slower moving form (AKP-2B), and thus in the 1:1 ratio expected if a single locus is responsible for the strain differences. Comparisons of Rl strains showed linkage of the Akp-2 locus to two loci on chromosome 4 as follows: centromere-Gpd-1-5.7 +/- 1.9 cM-Akp-2-0.6 +/- 0.4 cM-Ahd-1. These data based on 82 Rl strains typed for both the first and second loci, and 86 for the second and third loci.