Literature DB >> 7325969

Partial characterization of the carbohydrate units of rat intestinal sucrase--isomaltase.

A Herscovics, A Quaroni, B Bugge, K Kirsch.   

Abstract

Sucrase--isomaltase was purified from rat intestinal microvillus membranes after injection of D-[2-3H]mannose and L-[6-3H]fucose, using a column of monoclonal antibody-protein A-Sepharose. After Pronase digestion and gel filtration of the glycopeptides labelled from both precursors, a major part of the radioactivity was recovered in asparagine-linked complex oligosaccharides, and a smaller amount in partially alkali-labile high-molecular-weight glycopeptides. Only a small amount of [3H]mannose was found in endo-beta-N-acetylglucosaminidase H-sensitive high-mannose oligosaccharides.

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Year:  1981        PMID： 7325969      PMCID： PMC1163153          DOI： 10.1042/bj1970511

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

Authors:  P L Ey; S J Prowse; C R Jenkin
Journal:  Immunochemistry       Date:  1978-07

2.  Biogenesis of intestinal plasma membrane: posttranslational route and cleavage of sucrase-isomaltase.

Authors:  H P Hauri; A Quaroni; K J Isselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

3.  A simplified procedure for the isolation of the sucrase-isomaltase complex from rabbit intestine. Its amino-acid and sugar composition.

Authors:  A Cogoli; H Mosimann; C Vock; A K von Balthazar; G Semenza
Journal:  Eur J Biochem       Date:  1972-10-17

4.  Blood group antigenicity of purified human intestinal disaccharidases.

Authors:  J J Kelly; D H Alpers
Journal:  J Biol Chem       Date:  1973-12-10       Impact factor: 5.157

5.  The mode of association of the enzyme complex sucrase.isomaltase with the intestinal brush border membrane.

Authors:  J Brunner; H Hauser; H Braun; K J Wilson; H Wacker; B O'Neill; G Semenza
Journal:  J Biol Chem       Date:  1979-03-25       Impact factor: 5.157

6.  The hydrophobic anchor of small-intestinal sucrase--isomaltase: N-terminal sequence of isomaltase subunit.

Authors:  G Frank; J Brunner; H Hauser; H Wacker; G Semenza; H Zuber
Journal:  FEBS Lett       Date:  1978-12-01       Impact factor: 4.124

7.  Incomplete intracellular forms of intestinal surface membrane sucrase-isomaltase.

Authors:  J P Cezard; K A Conklin; B C Das; G M Gray
Journal:  J Biol Chem       Date:  1979-09-25       Impact factor: 5.157

8.  A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems.

Authors:  M Kessler; O Acuto; C Storelli; H Murer; M Müller; G Semenza
Journal:  Biochim Biophys Acta       Date:  1978-01-04

9.  Erythroglycan, a high molecular weight glycopeptide with the repeating structure [galactosyl-(1 leads to 4)-2-deoxy-2-acetamido-glucosyl(1 leads to 3)] comprising more than one-third of the protein-bound carbohydrate of human erythrocyte stroma.

Authors:  J Järnefelt; J Rush; Y T Li; R A Laine
Journal:  J Biol Chem       Date:  1978-11-25       Impact factor: 5.157

10.  Characterization of glycopeptides labelled from D-[2-3H]mannose and L-[6-3H]fucose in intestinal epithelial cell membranes during differentiation.

Authors:  A Herscovics; B Bugge; A Quaroni; K Kirsch
Journal:  Biochem J       Date:  1980-10-15       Impact factor: 3.857
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  2 in total

1.  Changes in intestinal absorption of nutrients and brush border glycoproteins after total parenteral nutrition in rats.

Authors:  S Miura; S Tanaka; M Yoshioka; H Serizawa; H Tashiro; H Shiozaki; H Imaeda; M Tsuchiya
Journal:  Gut       Date:  1992-04       Impact factor: 23.059

2.  Sucrase-isomaltase deficiency in humans. Different mutations disrupt intracellular transport, processing, and function of an intestinal brush border enzyme.

Authors:  H Y Naim; J Roth; E E Sterchi; M Lentze; P Milla; J Schmitz; H P Hauri
Journal:  J Clin Invest       Date:  1988-08       Impact factor: 14.808
  2 in total

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