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Literature DB >> 7321099

Transformation-defective mutant of adenovirus type 5 containing a single altered E1a mRNA species.

Abstract

A mutant of adenovirus type 5 containing an octanucleotide insert in region E1a of the viral genome was constructed. The insert was present in only one (13s) of the three overlapping mRNA's synthesized from this region. The insert was within the sequences removed by RNA splicing during the production of the other two nRNA's. The insertion resulted in a shift in the translational reading frame of the 13s mRNA and the probable premature termination of translation. The mutant was defective for viral DNA replication in HeLa cells and the transformation of rat embryo and baby rat kidney cells, indicating that a product encoded by the 13s nRNA is required for these two processes. Other early regions of the genome were expressed in HeLa cells infected by this mutant although in some cases the expression was decreased as compared with wild-type-infected cells.

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Year: 1981 PMID： 7321099 PMCID： PMC256677

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

26 in total

1. Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cells.

Authors: S J Flint; P H Gallimore; P A Sharp
Journal: J Mol Biol Date: 1975-07-25 Impact factor: 5.469

2. Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40.

Authors: F L Graham; P J Abrahams; C Mulder; H L Heijneker; S O Warnaar; F A De Vries; W Fiers; A J Van Der Eb
Journal: Cold Spring Harb Symp Quant Biol Date: 1975

3. Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5.

Authors: J Sambrook; M Botchan; P Gallimore; B Ozanne; U Pettersson; J Williams; P A Sharp
Journal: Cold Spring Harb Symp Quant Biol Date: 1975

4. Viral nucleic acid sequences in transformed cells. IV. A study of the sequences of adenovirus 5 DNA and RNA in four lines of adenovirus 5-transformed rodent cells using specific fragments of the viral genome.

Authors: S J Flint; J Sambrook; J F Williams; P A Sharp
Journal: Virology Date: 1976-07-15 Impact factor: 3.616

5. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

6. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;.

Authors: P H Gallimore
Journal: J Mol Biol Date: 1974-10-15 Impact factor: 5.469

7. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome.

Authors: P A Sharp; U Pettersson; J Sambrook
Journal: J Mol Biol Date: 1974-07-15 Impact factor: 5.469

8. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

9. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.

Authors: T Harrison; F Graham; J Williams
Journal: Virology Date: 1977-03 Impact factor: 3.616

10. Proteins and messenger RNAs of the transforming region of wild-type and mutant adenoviruses.

Authors: H Esche; M B Mathews; J B Lewis
Journal: J Mol Biol Date: 1980-09-25 Impact factor: 5.469

58 in total

1. Adenovirus infection of differentiated F9 cells results in a global shut-off of differentiation-induced gene expression.

Authors: R J Weigel; J R Nevins
Journal: Nucleic Acids Res Date: 1990-10-25 Impact factor: 16.971

2. Genetic dissection of the transactivating domain of the E1a 289R protein of adenovirus type 2.

Authors: M L Fahnestock; J B Lewis
Journal: J Virol Date: 1989-04 Impact factor: 5.103

Review 3. Expression and interactions of human adenovirus oncoproteins.

Authors: P A Boulanger; G E Blair
Journal: Biochem J Date: 1991-04-15 Impact factor: 3.857

4. The adenovirus Ela gene induces differentiation of F9 teratocarcinoma cells.

Authors: X Montano; D P Lane
Journal: Mol Cell Biol Date: 1987-05 Impact factor: 4.272

5. p300 binding by E1A cosegregates with p53 induction but is dispensable for apoptosis.

Authors: S K Chiou; E White
Journal: J Virol Date: 1997-05 Impact factor: 5.103

6. Adenovirus E3-early promoter: sequences required for activation by E1A.

Authors: D L Weeks; N C Jones
Journal: Nucleic Acids Res Date: 1985-07-25 Impact factor: 16.971

7. Identification of separate domains in the adenovirus E1A gene for immortalization activity and the activation of virus early genes.

Authors: E Moran; B Zerler; T M Harrison; M B Mathews
Journal: Mol Cell Biol Date: 1986-10 Impact factor: 4.272

8. Individual adenovirus type 5 early region 1A gene products elicit distinct alterations of cellular morphology and gene expression.

Authors: B E Roberts; J S Miller; D Kimelman; C L Cepko; I R Lemischka; R C Mulligan
Journal: J Virol Date: 1985-11 Impact factor: 5.103

9. Synthesis in Escherichia coli of human adenovirus type 12 transforming proteins encoded by early region 1A 13S mRNA and 12S mRNA.

Authors: D Kimelman; L A Lucher; K H Brackmann; J S Symington; M Ptashne; M Green
Journal: Proc Natl Acad Sci U S A Date: 1984-10 Impact factor: 11.205

10. Production of a monospecific antiserum against the early region 1A proteins of adenovirus 12 and adenovirus 5 by an adenovirus 12 early region 1A-beta-galactosidase fusion protein antigen expressed in bacteria.

Authors: M O Scott; D Kimelman; D Norris; R P Ricciardi
Journal: J Virol Date: 1984-06 Impact factor: 5.103