Reversed-phase high-pressure liquid chromatographic analysis of sulconazole in plasma.

A sensitive and specific analytical method for the measurement of sulconazole in plasma is described. The compound was extracted from plasma at pH 10 with hexane-methylene chloride. Samples were subjected to high-pressure liquid chromatography (HPLC) using an acetonitrile-phosphate buffer mixture as the mobile phase. The components of interest were measured using a variable-wavelength detector at 229 nm. Sulconazole concentrations of greater than or equal to 0.5 microgram/ml can be measured with confidence using this method. Linear calibration curves were constructed over the concentration range of 0.5-5 microgram/ml for sulconazole from dog plasma. A dog was administered a single oral 1000-mg dose of tritiated sulconazole nitrate; total plasma radioactivity and sulconazole plasma levels determined by HPLC are reported.