High-performance liquid chromatographic separation, isolation and identification of 1,2,3-thiadiazole-5-carboxaldoxime glucuronide in rabbit urine.

Reversed-phase high-performance liquid chromatography was used to separate and isolate the glucuronic acid conjugate of 1,2,3-thiadiazole-5-carboxaldoxime from urine of rabbits after intravenous injection of the oxime. The conjugate was identified by gas chromatography-mass spectrometry as its trimethylsilylated methyl ester and by nuclear magnetic resonance spectrometry. Additional information was obtained from thin-layer chromatography and high-voltage paper electrophoresis.