Literature DB >> 7317005

Identification of Limulus polyphemus haemocyanin messenger RNA.

E J Wood, J Bonaventura.   

Abstract

Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

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Year:  1981        PMID： 7317005      PMCID： PMC1163044          DOI： 10.1042/bj1960653

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Cell-free synthesis of a prolactin precursor directed by mRNA from cultured rat pituitary cells.

Authors:  G A Evans; M G Rosenfeld
Journal:  J Biol Chem       Date:  1976-05-10       Impact factor: 5.157

2.  Identification of hemocyanin in the cyanocytes of Carcinus maenas.

Authors:  A Ghiretti-Magaldi; C Milanesi; B Salvato
Journal:  Experientia       Date:  1973-10-15

3.  The branchial gland: a site of haemocyanin synthesis in Octopus.

Authors:  P N Dilly; J B Messenger
Journal:  Z Zellforsch Mikrosk Anat       Date:  1972

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

5.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

6.  Identification of the putative cell-free translation product of rat calcitonin mRNA.

Authors:  S G Amara; M G Rosenfeld; R S Birnbaum; B A Roos
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

7.  Hemocyanin of the horseshoe crab, Limulus polyphemus. Structural differentiation of the isolated components.

Authors:  B Sullivan; J Bonaventura; C Bonaventura; G Godette
Journal:  J Biol Chem       Date:  1976-12-10       Impact factor: 5.157

8.  Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.

Authors:  G Blobel; B Dobberstein
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539

9.  Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Authors:  G Blobel; B Dobberstein
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539

10.  The cyanoblast: hemocyanin formation in Limulus polyphemus.

Authors:  W H Fahrenbach
Journal:  J Cell Biol       Date:  1970-02       Impact factor: 10.539
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  2 in total

1.  Replacement of a cytosolic copper/zinc superoxide dismutase by a novel cytosolic manganese superoxide dismutase in crustaceans that use copper (haemocyanin) for oxygen transport.

Authors:  Marius Brouwer; Thea Hoexum Brouwer; Walter Grater; Nancy Brown-Peterson
Journal:  Biochem J       Date:  2003-08-15       Impact factor: 3.857

2.  Prekeratin biosynthesis in human scalp epidermis.

Authors:  P T Bladon; P E Bowden; W J Cunliffe; E J Wood
Journal:  Biochem J       Date:  1982-10-15       Impact factor: 3.857
  2 in total

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