Literature DB >> 7312634

Is Tb3+ fluorescence enhancement only due to binding to single stranded polynucleotides.

T Haertlé, J Augustyniak, W Guschlbauer.   

Abstract

Enhancement of Tb3+ fluorescence upon binding to double-stranded ribo- and deoxyribo-duplexes was investigated. It was observed that certain double stranded ribopolynucleotides completely quenched the Tb3+ fluorescence and others did not. It is concluded that the nature of the base in the duplex is critical for this enhancement. - Polydeoxyduplexes also showed enhancement of Tb3+ fluorescence, but much higher terbium concentrations were necessary to obtain similar fluorescence signals, indicative of unspecific effects. CD spectra evidence considerable conformational changes of these duplexes, in particular poly(dG-C) . poly(dG-C( which assumes the Z-form in 0.1 nM Tb3+.

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Year:  1981        PMID： 7312634      PMCID： PMC327593          DOI： 10.1093/nar/9.22.6191

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  A crystallographic study of metal-binding to yeast phenylalanine transfer RNA.

Authors:  A Jack; J E Ladner; D Rhodes; R S Brown; A Klug
Journal:  J Mol Biol       Date:  1977-04-15       Impact factor: 5.469

2.  Binding of lanthanides and of divalent metal ions to porcine trypsin.

Authors:  M Epstein; A Levitzki; J Reuben
Journal:  Biochemistry       Date:  1974-04-09       Impact factor: 3.162

3.  Conformation and reactivity of DNA. VI. Circular dichroism studies of salt-induced conformational changes of DNAs of different base composition.

Authors:  C Zimmer; G Luck
Journal:  Biochim Biophys Acta       Date:  1974-08-15

4.  Fluorescence of nucleic acid-terbium (3) complexes.

Authors:  C Formoso
Journal:  Biochem Biophys Res Commun       Date:  1973-08-21       Impact factor: 3.575

5.  Different conformations of double-stranded nucleic acid in solution as revealed by circular dichroism.

Authors:  V I Ivanov; L E Minchenkova; A K Schyolkina; A I Poletayev
Journal:  Biopolymers       Date:  1973       Impact factor: 2.505

6.  Europium as a fluorescent probe of transfer RNA structure.

Authors:  J M Wolfson; D R Kearns
Journal:  Biochemistry       Date:  1975-04-08       Impact factor: 3.162

7.  Determination of the interaction of ADP and dADP with copper(II), manganese(II) and lanthanide(III) ions by nuclear-magnetic-resonance spectroscopy.

Authors:  G V Fazakerley; D G Reid
Journal:  Eur J Biochem       Date:  1979-02-01

8.  Fluorescence of terbium ion-nucleic acid complexes: a sensitive specific probe for unpaired residues in nucleic acids.

Authors:  M D Topal; J R Fresco
Journal:  Biochemistry       Date:  1980-11-25       Impact factor: 3.162

9.  Lanthanide fluorescence studies of transfer RNAf(met) conformation.

Authors:  D Pavlick; C Formoso
Journal:  Biochemistry       Date:  1978-04-18       Impact factor: 3.162
  9 in total
  4 in total

1.  Liquid crystal dispersions of DNA complexes with gadolinium as a possible basis for neutron-trapping therapy.

Authors:  Yu M Yevdokimov; V I Salyanov; O V Kondrashina; M A Lagutina; A A Gasanov; V N Nikiforov; V I Borshchevskii; K A Dembo; I V Reshetov
Journal:  Dokl Biochem Biophys       Date:  2005 May-Jun       Impact factor: 0.788

2.  SAXS-data-based structural modeling of DNA-gadolinium complexes fixed in particles of cholesteric liquid-crystalline dispersions.

Authors:  E V Shtykova; V V Volkov; V I Salyanov; Yu M Yevdokimov
Journal:  Eur Biophys J       Date:  2010-03-02       Impact factor: 1.733

3.  Conformational transitions of poly(dA-dC).poly(dG-dT) induced by high salt or in ethanolic solution.

Authors:  C Zimmer; S Tymen; C Marck; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1982-02-11       Impact factor: 16.971

4.  The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA.

Authors:  A Woisard; W Guschlbauer; G V Fazakerley
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971
  4 in total

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