Literature DB >> 7311108

Separation of Clostridium botulinum type A derivative toxin into two fragments.

S Kozaki, S Togashi, G Sakaguchi.   

Abstract

Two fragments with molecular weights of 105,000 (fragment I) and 58,000 (fragment II) were separated chromatographically from each other after Clostridium botulinum type A derivative toxin adsorbed onto a QAE-Sephadex column was treated with dithiothreitol and urea. They were antigenic and formed crossing precipitin lines against anti-derivative toxin in agar gel diffusion tests. Upon removal of dithiothreitol and urea by dialysis, the two fragments reassembled to reconstruct the derivative toxin molecule.

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Year:  1981        PMID： 7311108     DOI： 10.7883/yoken1952.34.61

Source DB:  PubMed          Journal:  Jpn J Med Sci Biol        ISSN： 0021-5112


  10 in total

1.  Immunological characterization of papain-induced fragments of Clostridium botulinum type A neurotoxin and interaction of the fragments with brain synaptosomes.

Authors:  S Kozaki; A Miki; Y Kamata; J Ogasawara; G Sakaguchi
Journal:  Infect Immun       Date:  1989-09       Impact factor: 3.441

2.  The use of monoclonal antibodies to analyze the structure of Clostridium botulinum type E derivative toxin.

Authors:  S Kozaki; Y Kamata; T Nagai; J Ogasawara; G Sakaguchi
Journal:  Infect Immun       Date:  1986-06       Impact factor: 3.441

3.  Neurotransmitter release is blocked intracellularly by botulinum neurotoxin, and this requires uptake of both toxin polypeptides by a process mediated by the larger chain.

Authors:  B Poulain; L Tauc; E A Maisey; J D Wadsworth; P M Mohan; J O Dolly
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205

4.  Mapping of the antibody-binding regions on botulinum neurotoxin H-chain domain 855-1296 with antitoxin antibodies from three host species.

Authors:  M Z Atassi; B Z Dolimbek; M Hayakari; J L Middlebrook; B Whitney; M Oshima
Journal:  J Protein Chem       Date:  1996-10

5.  Structure of heavy and light chain subunits of type A botulinum neurotoxin analyzed by circular dichroism and fluorescence measurements.

Authors:  B R Singh; B R DasGupta
Journal:  Mol Cell Biochem       Date:  1989-01-23       Impact factor: 3.396

6.  Processing of tetanus and botulinum A neurotoxins in isolated chromaffin cells.

Authors:  E Erdal; F Bartels; T Binscheck; G Erdmann; J Frevert; A Kistner; U Weller; J Wever; H Bigalke
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1995-01       Impact factor: 3.000

7.  Neuritogenic actions of botulinum neurotoxin A on cultured motor neurons.

Authors:  Julie A Coffield; Xiuzhen Yan
Journal:  J Pharmacol Exp Ther       Date:  2009-04-16       Impact factor: 4.030

8.  Reductive methylation of lysine residues of botulinum neurotoxin types A and B.

Authors:  V Sathyamoorthy; B R DasGupta
Journal:  Mol Cell Biochem       Date:  1988-09       Impact factor: 3.396

9.  Interaction of 125I-labeled botulinum neurotoxins with nerve terminals. II. Autoradiographic evidence for its uptake into motor nerves by acceptor-mediated endocytosis.

Authors:  J D Black; J O Dolly
Journal:  J Cell Biol       Date:  1986-08       Impact factor: 10.539

10.  Mapping of the antibody-binding regions on the HN-domain (residues 449-859) of botulinum neurotoxin A with antitoxin antibodies from four host species. Full profile of the continuous antigenic regions of the H-chain of botulinum neurotoxin A.

Authors:  M Zouhair Atassi; Behzod Z Dolimbek
Journal:  Protein J       Date:  2004-01       Impact factor: 4.000
  10 in total

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