Literature DB >> 7310384

Plaque assay for titration of bovine enteric coronavirus.

J F Vautherot.   

Abstract

The plaquing ability of two isolates of bovine enteric coronavirus (BECV) was studied in HRT18 (human rectal adenocarcinoma) cell monolayers. Both isolates were able to induce plaque formation within 2 to 3 days; plaques appeared as round opalescent areas which remained colourless after neutral red or crystal violet staining. A good correlation was found between the titres as determined either by counting the plaques that were visible to the naked eye before and after neutral red staining, or by enumerating fluorescence or haemadsorption foci.

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Year:  1981        PMID： 7310384     DOI： 10.1099/0022-1317-56-2-451

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  9 in total

1.  Radioactive and enzymatic cloned cDNA probes for bovine enteric coronavirus detection by molecular hybridization.

Authors:  J Collomb; C Finance; S Alabouch; J Laporte
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

2.  Isolation of coronaviruses antigenically indistinguishable from bovine coronavirus from wild ruminants with diarrhea.

Authors:  H Tsunemitsu; Z R el-Kanawati; D R Smith; H H Reed; L J Saif
Journal:  J Clin Microbiol       Date:  1995-12       Impact factor: 5.948

3.  Propagation of the virus of porcine epidemic diarrhea in cell culture.

Authors:  M Hofmann; R Wyler
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

4.  A serological comparison of bovine coronavirus strains.

Authors:  A A el-Ghorr; D R Snodgrass; F M Scott; I Campbell
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

5.  Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV).

Authors:  M Hofmann; R Wyler
Journal:  Vet Microbiol       Date:  1989-06       Impact factor: 3.293

6.  Phage-displayed peptides having antigenic similarities with porcine epidemic diarrhea virus (PEDV) neutralizing epitopes.

Authors:  Deu John M Cruz; Chul-Joong Kim; Hyun-Jin Shin
Journal:  Virology       Date:  2006-09-01       Impact factor: 3.616

7.  Application of a focus formation assay for detection and titration of porcine epidemic diarrhea virus.

Authors:  Deu John M Cruz; Hyun-Jin Shin
Journal:  J Virol Methods       Date:  2007-06-18       Impact factor: 2.014

Review 8.  Bovine coronavirus.

Authors:  M A Clark
Journal:  Br Vet J       Date:  1993 Jan-Feb

9.  Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability.

Authors:  Ching-Hung Lin; Cheng-Yao Yang; Meilin Wang; Shan-Chia Ou; Chen-Yu Lo; Tsung-Lin Tsai; Hung-Yi Wu
Journal:  Cells       Date:  2020-10-19       Impact factor: 6.600
  9 in total

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