Preparation and characterization of cobalt(II)-substituted bovine serum amine oxidase.

The Type-2 copper ion in bovine serum amine oxidase (BSAO) was first replaced by cobalt(II) ion. The enzymatic activity of Co(II)BSAO was 13.3% of that of native BSAO. The various spectral data indicated that the Co(II) center has tetrahedral geometry (high-spin state) and is linked by two nitrogens and two oxygens. It was also found that the putative organic chromophore suggested by many investigators exhibits a positive CD band near 370 nm and a negative CD band near 440 nm.