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Literature DB >> 7306033

Reconstruction of the smaller subparticle of rabbit ribosomes from core-particle and split-protein fractions.

Abstract

The smaller subparticle of rabbit reticulocyte ribosomes was shown to yield core-particle and split-protein fractions on treatment with 2.5 M-NH4Cl/61 mM-MgCl2. The core-particle fraction was inactive in poly(U)-directed polyphenylalanine synthesis, but activity was restored after recombination with the split-protein fraction. Optimum ionic conditions for the reconstruction of active subparticles were found to be 0.75 M-NH4Cl/19 mM-MgCl2 at 0 degrees C. Improved extents of reconstruction were obtained when the core-particles were isolated by methods that avoided pelleting. Core-particles isolated from subparticles pretreated with either proteinases or ribonucleases had diminished capacity to become re-activated.

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Year: 1981 PMID： 7306033 PMCID： PMC1162830 DOI： 10.1042/bj1940931

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

1. Study on mammalian ribosomal protein reactivity in situ. III. Effect of trypsin on 40S and 60S subunits.

Authors: M Arpin; J P Reboud; A M Reboud
Journal: Biochimie Date: 1975 Impact factor: 4.079

2. A new two-dimensional gel electrophoresis system for the analysis of complex protein mixtures: application to the ribosome of E. coli.

Authors: U C Knopf; A Sommer; J Kenny; R R Traut
Journal: Mol Biol Rep Date: 1975-03 Impact factor: 2.316

Review 3. Assembly of bacterial ribosomes.

Authors: M Nomura
Journal: Science Date: 1973-03-02 Impact factor: 47.728

8. Differences in electrophoretic behaviour of eight ribosomal proteins from rat and rabbit tissues and evidence for proteolytic action on liver proteins.

Authors: J J Madjar; R R Traut
Journal: Mol Gen Genet Date: 1980

9. Studies on the small subunit of rat liver ribosomes: some biochemical properties with specific references to the reconstruction of the small subunit.

Authors: K Terao; K Ogata
Journal: Biochim Biophys Acta Date: 1971-12-16

10. The function of high-molecular-weight ribonucleic acid from rabbit reticulocytes in haemoglobin biosynthesis.

Authors: H R Arnstein; R A Cox; J A Hunt
Journal: Biochem J Date: 1964-09 Impact factor: 3.857

4 in total

1. Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.

Authors: J Wu; D R Beniac; G Harauz
Journal: Mol Cell Biochem Date: 1995-02-09 Impact factor: 3.396

Reconstruction of the smaller subparticle of rabbit ribosomes from core-particle and split-protein fractions.

1. Study on mammalian ribosomal protein reactivity in situ. III. Effect of trypsin on 40S and 60S subunits.

2. A new two-dimensional gel electrophoresis system for the analysis of complex protein mixtures: application to the ribosome of E. coli.

Review 3. Assembly of bacterial ribosomes.

4. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.

5. Partial in vitro reconstitution of active 40S ribosomal subunits from rat liver.

6. Incorporating activity of ribosomes and integrity of ribosomal RNA.

7. Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.

8. Differences in electrophoretic behaviour of eight ribosomal proteins from rat and rabbit tissues and evidence for proteolytic action on liver proteins.

9. Studies on the small subunit of rat liver ribosomes: some biochemical properties with specific references to the reconstruction of the small subunit.

10. The function of high-molecular-weight ribonucleic acid from rabbit reticulocytes in haemoglobin biosynthesis.

1. Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.

2. Partial reconstitution of 60S ribosomal subunits from yeast.

3. Modification of 40S ribosomal subunits from yeast with dimethylmaleic anhydride.

4. Disassembly and reconstitution of yeast 60S ribosomal subunits.