Literature DB >> 7305967

Tritiation of alpha-bungarotoxin with N-succinimidyl [2,3-3H]propionate. A useful reagent for labelling proteins.

J O Dolly, E A Nockles, M M Lo, E A Barnard.   

Abstract

Mono[3H]propionyl-alpha-bungarotoxin, prepared with N-succinimidyl [2,3-3H]propionate (sp. radioactivity 50 Ci/mmol) and purified to homogeneity by electrofocusing, retains its biological activity and stability. Rate constants for its binding to acetylcholine receptor were 4.4-fold lower than for unlabelled toxin; no dissociation was detectable. Analysis of enzymic digests of toxin showed 3H is located mainly of entirely in epsilon-propionyl-lysine.

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Year:  1981        PMID： 7305967      PMCID： PMC1162686          DOI： 10.1042/bj1930919

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

Review 1.  The acetylcholine receptor and the ionic conductance modulation system of skeletal muscle.

Authors:  E A Barnard; J O Dolly; C W Porter; E X Albuquerque
Journal:  Exp Neurol       Date:  1975-07       Impact factor: 5.330

2.  Purification and characterization of an acetylcholine receptor from mammalian skeletal muscle.

Authors:  J O Dolly; E A Barnard
Journal:  Biochemistry       Date:  1977-11-15       Impact factor: 3.162

3.  Chromatographic separation of the venom of Bungarus multicinctus and characterization of its components.

Authors:  C Y Lee; S L Chang; S T Kau; S H Luh
Journal:  J Chromatogr       Date:  1972-10-05

4.  Binding of perhydro-histrionicotoxin to the postsynaptic membrane of skeletal muscle in relation to its blockage of acetylcholine-induced depolarization.

Authors:  J O Dolly; E X Albuquerque; J Sarvey; B Mallick; E A Barnard
Journal:  Mol Pharmacol       Date:  1977-01       Impact factor: 4.436

5.  Three-dimensional structure of the "long" neurotoxin from cobra venom.

Authors:  M D Walkinshaw; W Saenger; A Maelicke
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

6.  Complete purification of beta-bungarotoxin. Characterization of its action and that of tityustoxin on synaptosomal accumulation and release of acetylcholine.

Authors:  J W Spokes; J O Dolly
Journal:  Biochim Biophys Acta       Date:  1980-02-15

7.  Preparation and characterization of 3H-labeled alpha-bungarotoxin.

Authors:  S W Jones; W R Thompson
Journal:  Anal Biochem       Date:  1980-01-15       Impact factor: 3.365

8.  Cholinergic receptor molecules and cholinesterase molecules at mouse skeletal muscle junctions.

Authors:  E A Barnard; J Wieckowski; T H Chiu
Journal:  Nature       Date:  1971-11-26       Impact factor: 49.962

9.  Fluctuations in proline and other free amino acids during the mitotic cycle of the myxomycete Physarum polycephalum.

Authors:  R J Threlfall; A J Thomas
Journal:  Eur J Biochem       Date:  1979-01-02
  9 in total
  4 in total

1.  Uptake and accumulation of tritiated uteroglobin by day-6 rabbit blastocysts.

Authors:  D R Dannhorn; C Kirchner
Journal:  Cell Tissue Res       Date:  1990-12       Impact factor: 5.249

2.  Uptake of tritiated uteroglobin by the endometrium of the rabbit during peri-implantation.

Authors:  D R Dannhorn; C Kirchner
Journal:  Cell Tissue Res       Date:  1990-03       Impact factor: 5.249

3.  Tetanus toxin-[2,3-3H]propionamid. Synthesis, specific activity, immunoreactivity, toxicity and ascent into the spinal cord.

Authors:  T B Meyer-Eppler; H H Wellhöner
Journal:  Arch Toxicol       Date:  1983-04       Impact factor: 5.153

4.  Saposin B-dependent reconstitution of arylsulfatase A activity in vitro and in cell culture models of metachromatic leukodystrophy.

Authors:  Ulrich Matzner; Bernadette Breiden; Günter Schwarzmann; Afshin Yaghootfam; Arvan L Fluharty; Andrej Hasilik; Konrad Sandhoff; Volkmar Gieselmann
Journal:  J Biol Chem       Date:  2009-02-18       Impact factor: 5.157
  4 in total

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