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Literature DB >> 7305950

Calcium-binding constants of trypsin and trypsinogen. A reassessment.

Abstract

The Ca2+-binding constants for trypsin and trypsinogen have been reassessed by using enzyme that has been purified by affinity chromatography and measuring the distribution of 45Ca2+ between the protein and a cation exchanger. The pKCa2+ value of 4.5 for the high-affinity site on trypsin was 1 logarithmic unit greater than that previously reported.

Entities: Chemical

Mesh：

Substances：

Year: 1981 PMID： 7305950 PMCID： PMC1162647 DOI： 10.1042/bj1930655

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

14 in total

1. Dissociation constants of radium-organic acid complexes measured by ion exchange.

Authors: J SCHUBERT; E R RUSSELL; L S MYERS
Journal: J Biol Chem Date: 1950-07 Impact factor: 5.157

2. Binding of lanthanides and of divalent metal ions to porcine trypsin.

Authors: M Epstein; A Levitzki; J Reuben
Journal: Biochemistry Date: 1974-04-09 Impact factor: 3.162

3. Calcium-binding protein and calcium absorption after vitamin D administration.

Authors: J Harmeyer; H F Deluca
Journal: Arch Biochem Biophys Date: 1969-09 Impact factor: 4.013

4. Vitamin D-dependent calcium-binding protein. Purification and some properties.

Authors: R H Wasserman; R A Corradino; A N Taylor
Journal: J Biol Chem Date: 1968-07-25 Impact factor: 5.157

5. Affinity chromatography: purification of bovine trypsin and thrombin.

Authors: H F Hixson; A H Nishikawa
Journal: Arch Biochem Biophys Date: 1973-02 Impact factor: 4.013

6. A second activation peptide from bovine cationic trypsinogen.

Authors: D Webster; R E Offord
Journal: Biochem J Date: 1977-10-01 Impact factor: 3.857

7. The mechanism of activation of trypsinogen. The role of the four N-terminal aspartyl residues.

Authors: J P Abita; M Delaage; M Lazdunski
Journal: Eur J Biochem Date: 1969-04

8. The location of the calcium ion binding site in bovine alpha-trypsin and beta-trypsin using lanthanide ion probes.

Authors: F Abbott; J E Gomez; E R Birnbaum; D W Darnall
Journal: Biochemistry Date: 1975-11-04 Impact factor: 3.162

9. The binding of Ca2+ to trypsinogen and its relation to the mechanism of activation.

Authors: M Delaage; M Lazdunski
Journal: Biochem Biophys Res Commun Date: 1967-08-07 Impact factor: 3.575

10. On the activation of trypsinogen. A study of peptide models related to the N-terminal sequence of the zymogen.

Authors: M Delaage; P Desnuelle; M Lazdunski; E Bricas; J Savrda
Journal: Biochem Biophys Res Commun Date: 1967-10-26 Impact factor: 3.575

2 in total

1. Modelling of the serine-proteinase fold by X-ray and neutron scattering and sedimentation analyses: occurrence of the fold in factor D of the complement system.

Authors: S J Perkins; K F Smith; J M Kilpatrick; J E Volanakis; R B Sim
Journal: Biochem J Date: 1993-10-01 Impact factor: 3.857

2. Influence of proteolytic enzymes and calcium-binding agents on nuclear and cell surface topography.

Authors: J Thyberg; S Moskalewski
Journal: Cell Tissue Res Date: 1984 Impact factor: 5.249

2 in total