Literature DB >> 7305926

Properties of methyl acetimidate and its use as a protein-modifying reagent.

A J Makoff, A D Malcolm.   

Abstract

The rate of hydrolysis of the imido ester methyl acetimidate and its rate of amidination of denatured aldolase were investigated under different conditions of temperature, pH and ionic strength. Both rate constants increase greatly with temperature, whereas ionic strength has no effect on either. The effect of pH is more complex. Between pH 6.8 and 8.8 the rate of hydrolysis decreases and the rate of amidination increases. These results are discussed in terms of the reaction mechanisms involved.

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Year:  1981        PMID： 7305926      PMCID： PMC1162596          DOI： 10.1042/bj1930245

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  4 in total

1.  Formation of non-amidine products in the reaction of primary amines with imido esters.

Authors:  D T Browne; S B Kent
Journal:  Biochem Biophys Res Commun       Date:  1975-11-03       Impact factor: 3.575

2.  Formation of non-amidine products in the chemical modification of horse liver alcohol dehydrogenase with imido esters.

Authors:  D T Browne; S B Kent
Journal:  Biochem Biophys Res Commun       Date:  1975-11-03       Impact factor: 3.575

3.  Methods for obtaining peptide maps of proteins on a subnanomole scale.

Authors:  D L Bates; R N Perham; J R Coggins
Journal:  Anal Biochem       Date:  1975-09       Impact factor: 3.365

4.  Identification of a class of lysines within the non-specific DNA-binding site of RNA polymerase core enzyme from Escherichia coli.

Authors:  A J Makoff; A D Malcolm
Journal:  Eur J Biochem       Date:  1980-05
  4 in total
  8 in total

Review 1.  Utilization of the free energy of the reversible binding of protein and modifying agent towards the rate-enhancement of protein covalent modification.

Authors:  E T Rakitzis
Journal:  Biochem J       Date:  1990-08-01       Impact factor: 3.857

2.  Computer simulations of the kinetics of irreversible enzyme inhibition by an unstable inhibitor.

Authors:  C M Topham
Journal:  Biochem J       Date:  1986-12-15       Impact factor: 3.857

Review 3.  Kinetics of protein modification reactions.

Authors:  E T Rakitzis
Journal:  Biochem J       Date:  1984-01-15       Impact factor: 3.857

4.  Kinetics of irreversible enzyme inhibition by an unstable inhibitor.

Authors:  E T Rakitzis
Journal:  Biochem J       Date:  1981-11-01       Impact factor: 3.857

Review 5.  Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications.

Authors:  Xiaoran Roger Liu; Mengru Mira Zhang; Michael L Gross
Journal:  Chem Rev       Date:  2020-04-22       Impact factor: 60.622

6.  Activation of bovine tracheal chloride channels by amino group-specific reagents.

Authors:  M Duszyk; Y Shu; A K Ho; S F Man
Journal:  J Physiol       Date:  1998-10-01       Impact factor: 5.182

7.  Mechanisms of corneal tissue cross-linking in response to treatment with topical riboflavin and long-wavelength ultraviolet radiation (UVA).

Authors:  A Scott McCall; Stefan Kraft; Henry F Edelhauser; George W Kidder; Richard R Lundquist; Helen E Bradshaw; Zinaida Dedeic; Megan J C Dionne; Ethan M Clement; Gary W Conrad
Journal:  Invest Ophthalmol Vis Sci       Date:  2009-07-30       Impact factor: 4.799

Review 8.  Mass Spectrometry-Based Structural Proteomics for Metal Ion/Protein Binding Studies.

Authors:  Yanchun Lin; Michael L Gross
Journal:  Biomolecules       Date:  2022-01-15
  8 in total

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