Literature DB >> 7298392

Intermediate electron-acceptors in quantitative cytochemistry. Comparison of phenazine methosulphate and Meldola Blue.

B Henderson, N Loveridge.   

Abstract

The efficacy of Meldola Blue (MB), a new intermediate electron-acceptor, has been compared with that of phenazine methosulphate (PMS) in the assay of oxidoreductase activity in cryostat sections; various tetrazolium salts have been used as the final electron-acceptors. Three enzymes: succinate dehydrogenase, glucose 6-phosphate dehydrogenase and lactate dehydrogenase were investigated, the activity in sections being quantitated by scanning and integrating microdensitometry. Phenazine methosulphate was superior to Meldola Blue in transferring reducing equivalents from reduced coenzyme to all the tetrazolium salts examined.

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Year:  1981        PMID： 7298392     DOI： 10.1007/bf00493278

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  8 in total

1.  Histochemical localization of specific oxidative enzymes. V. The dissociation of succinic dehydrogenase from carriers by lipase and the specific histochemical localization of the dehydrogenase with phenazine methosulfate and tetrazolium salts.

Authors:  E FARBER; E BUEDING
Journal:  J Histochem Cytochem       Date:  1956-07       Impact factor: 2.479

2.  Phenazine compounds as carriers in the hexosemonophosphate system.

Authors:  F Dickens; H McIlwain
Journal:  Biochem J       Date:  1938-09       Impact factor: 3.857

3.  The use of Meldolablue in isozyme stains after electrophoresis.

Authors:  V S Turner; D A Hopkinson
Journal:  FEBS Lett       Date:  1979-09-15       Impact factor: 4.124

4.  Studies on succinate oxidation. I. The use of intact tissue sections.

Authors:  R G Butcher
Journal:  Exp Cell Res       Date:  1970-04       Impact factor: 3.905

5.  A comparison of dehydrogenase activities in tissue homogenates and tissue sections.

Authors:  F P Altmann
Journal:  Biochem J       Date:  1969-08       Impact factor: 3.857

Review 6.  Tetrazolium salts and formazans.

Authors:  F P Altman
Journal:  Prog Histochem Cytochem       Date:  1976

7.  Meldola blue: a new electron carrier for the histochemical demonstration of dehydrogenases (SDH, LDH, G-6-PDH).

Authors:  P Kugler; K H Wrobel
Journal:  Histochemistry       Date:  1978-12-29

8.  STUDIES ON BLOOD CELL METABOLISM : I. THE EFFECT OF METHYLENE BLUE AND OTHER DYES UPON THE OXYGEN CONSUMPTION OF MAMMALIAN AND AVIAN ERYTHROCYTES.

Authors:  G A Harrop; E S Barron
Journal:  J Exp Med       Date:  1928-07-31       Impact factor: 14.307
  8 in total
  3 in total

1.  Diffusion during dehydrogenase reactions: the effects of intermediate electron acceptors.

Authors:  R G Butcher; A W Evans
Journal:  Histochem J       Date:  1984-08

2.  Succinate dehydrogenase activity in cultured human skin fibroblasts and amniotic fluid cells. A methodological study.

Authors:  T L Hansen; H Andersen
Journal:  Histochemistry       Date:  1983

3.  Quantitative dehydrogenase histochemistry with exogenous electron carriers (PMS, MPMS, MB).

Authors:  P Kugler
Journal:  Histochemistry       Date:  1982
  3 in total

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