Literature DB >> 7296832

The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns.

N L Anderson, J Taylor, A E Scandora, B P Coulter, N G Anderson.   

Abstract

We describe here a computer system for the analysis of high-resolution two-dimensional gel-electrophoresis patterns, with some initial applications. The system (called TYCHO) comprises programs for image acquisition, background subtraction and smoothing, spot detection, gaussian spot modeling, and pattern matching and comparison. It is based on a conventional minicomputer, but makes extensive use of a high-speed array processor in the image-processing and -modeling steps. Used in concert with the ISO-DALT two-dimensional electrophoresis system (Anal. Biochem. 85:331-354, 1978), TYCHO allows quantitative measurement of hundreds of proteins in complex biological samples, and constitutes the initial data-reduction system required for work towards a Human Protein Index.

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Year:  1981        PMID： 7296832

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  13 in total

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Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

5.  Apparatus for direct counting of beta-rays from two-dimensional protein gels: measurement of changes in protein synthesis due to changes in density of Chinese hamster ovary cells.

Authors:  H L Anderson; T T Puck; E B Shera
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

6.  The effects of lectin transformation on cytoplasmic polyadenylated RNA from human lymphocytes.

Authors:  E McCairns; D Fahey; G E Muscat; P B Rowe
Journal:  Mol Cell Biochem       Date:  1983       Impact factor: 3.396

7.  Computer-based image analysis of one-dimensional electrophoretic gels used for the separation of DNA restriction fragments.

Authors:  A J Gray; D E Beecher; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

8.  Evidence for regulatory genes on mouse chromosome 7 that affect the quantitative expression of proteins in the fetal and newborn liver.

Authors:  C S Giometti; M A Gemmell; J Taylor; S L Tollaksen; R Angeletti; S Gluecksohn-Waelsch
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

9.  Expression of transfected mutant beta-actin genes: alterations of cell morphology and evidence for autoregulation in actin pools.

Authors:  J Leavitt; S Y Ng; U Aebi; M Varma; G Latter; S Burbeck; L Kedes; P Gunning
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272

10.  Tropomyosin isoform switching in tumorigenic human fibroblasts.

Authors:  J Leavitt; G Latter; L Lutomski; D Goldstein; S Burbeck
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272
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