Cultured embryonic retinae transplanted to rat brain: differentiation and formation of projections to host superior colliculus.

Retinae of rats on embryonic day 14 were placed in explant culture for 2-14 days prior to transplantation adjacent to the superior colliculus of newborn rats. In explant culture cell division and neuronal differentiation continued unabated. One month after transplantation host brains were examined for transplant survival, differentiation and formation of projections to the host brain. The cultured retinal transplants survived and developed a morphology typical of mature retina, with normal cell and fiber laminae present. HRP injections into the host superior colliculus labeled neurons in the ganglion cell layer of the transplant which closely resembled ganglion cells in vivo. A small number of transplants received lesions. Degeneration material was traced into the superior colliculus and pretectal nuclei confirming that the cultured transplants had projections appropriate for retina entering the host brain. These results correlate closely with those seen after transplantation of embryonic rat retinae that had not been cultured. Thus, a period of explant culture of up to two weeks does not affect the ability of embryonic retinal transplants to differentiate and form projections into the host brain.