Comparison of radioimmunoassay and thin layer chromatographic assay methods for estimation of plasma prednisolone concentrations.

Radioimmunoassay and thin layer chromatographic methods of assay for prednisolone in plasma have been compared. These methods are comparable in terms of ease, speed of execution, and cost. They yielded similar estimates of prednisolone concentration without important bias over the concentration range generally encountered in clinical practice and may be considered comparable for pharmacokinetic studies.