Literature DB >> 7288404

Purification of measles virus glycoproteins and their integration into artificial lipid membranes.

P Casali, J G Sissons, R S Fujinami, M B Oldstone.   

Abstract

We report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artificial lipid bilayers. The two viral glycoproteins, HA and F, were isolated in preparative amounts from disrupted purified virus by lentil lectin affinity chromatography. The proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent Nonidet P40 (NP40) for a dialysable one, octylglucoside, while the proteins were immobilized on the lectin column and (ii) co-dialysis of the eluted glycoproteins in octylglucoside with phosphatidylcholine. The resultant 'virosomes' had visible 'spikes' and possessed haemagglutinating activity. These measles virosomes should provide a useful reagent for studying immune responses to measles virus, independent of the immunosuppressive effects of the whole virus.

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Year:  1981        PMID： 7288404     DOI： 10.1099/0022-1317-54-1-161

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  7 in total

1.  Preparation of liposomes via detergent removal from mixed micelles by dilution. The effect of bilayer composition and process parameters on liposome characteristics.

Authors:  W Jiskoot; T Teerlink; E C Beuvery; D J Crommelin
Journal:  Pharm Weekbl Sci       Date:  1986-10-17

2.  Isolation of the measles virus hemagglutinin protein in a soluble form by protease digestion.

Authors:  T A Sato; M Enami; T Kohama
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

3.  Purified Epstein-Barr virus Mr 340,000 glycoprotein induces potent virus-neutralizing antibodies when incorporated in liposomes.

Authors:  J R North; A J Morgan; J L Thompson; M A Epstein
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

4.  Functional and structural interactions between measles virus hemagglutinin and CD46.

Authors:  O Nussbaum; C C Broder; B Moss; L B Stern; S Rozenblatt; E A Berger
Journal:  J Virol       Date:  1995-06       Impact factor: 5.103

5.  In vitro generation of human cytotoxic lymphocytes by virus. Viral glycoproteins induce nonspecific cell-mediated cytotoxicity without release of interferon.

Authors:  P Casali; J G Sissons; M J Buchmeier; M B Oldstone
Journal:  J Exp Med       Date:  1981-09-01       Impact factor: 14.307

6.  Viruses disrupt functions of human lymphocytes. Effects of measles virus and influenza virus on lymphocyte-mediated killing and antibody production.

Authors:  P Casali; G P Rice; M B Oldstone
Journal:  J Exp Med       Date:  1984-05-01       Impact factor: 14.307

Review 7.  Measles virus glycoprotein complex assembly, receptor attachment, and cell entry.

Authors:  C K Navaratnarajah; V H J Leonard; R Cattaneo
Journal:  Curr Top Microbiol Immunol       Date:  2009       Impact factor: 4.291
  7 in total

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