| Literature DB >> 7287653 |
K Hosaka, T Kikuchi, N Mitsuhida, A Kawaguchi.
Abstract
A rapid and sensitive spectrophotometric assay for free fatty acids using acyl-CoA synthetase and acyl-CoA oxidase is described. It is sensitive to as low as 5 nmol of free fatty acids, and the standard curve is linear up to 100 nmol. The assay consists of the measurement of H2O2 produced from free fatty acids by acyl-CoA synthetase and acyl-CoA oxidase. The quantity of H2O2 is determined by the absorbance at 550 nm in the presence of catalase and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT). This method shows a broad specificity to long-chain fatty acids and the recoveries of added fatty acids (C12-C18) are more than 90%. The presence or absence of serum components or Escherichia coli cell-free extracts has no significant effect on the recovery of added palmitic acid.Entities:
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Year: 1981 PMID： 7287653 DOI： 10.1093/oxfordjournals.jbchem.a133380
Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387