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Literature DB >> 7278514

Microsomal phosphatidylethanolamine methyltransferase: some physical and kinetic properties.

Abstract

Some physical and kinetic properties of the microsomal enzyme(s) that convert phosphatidylethanolamine to phosphatidylcholine in rat and guinea pig livers have been investigated. The pH optima of the reactions were 9.8, 9.3 and 9.5 for the first, second and third methylation reactions, respectively. Incomplete heat denaturation of the protein catalyzing the first reaction contrasts with inactivation at 60 C of the enzymes catalyzing the second and third methylations. The maximal velocity of the first reaction of the guinea pig liver enzyme is 48 pmol/min/mg protein, substantially less than exhibited rate-limiting reaction of the three step methylation sequence in rat liver, 114 pmol/min/mg. The affinity of the microsomal enzyme for S-adenosylmethionine is greater in rat liver (Km = 18.2 microM) than in guinea pig liver (Km = 302 microM).

Entities: Chemical Species

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Year: 1981 PMID： 7278514 DOI： 10.1007/BF02535052

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

26 in total

1. RATES OF REACTIONS INVOLVED IN PHOSPHATIDE SYNTHESIS IN LIVER AND SMALL INTESTINE OF INTACT RATS.

Authors: E M WISE; D ELWYN
Journal: J Biol Chem Date: 1965-04 Impact factor: 5.157

2. INFLUENCE OF THYROID HORMONES ON L-ALPHA-GLYCEROPHOSPHATE DEHYDROGENASES AND OTHER DEHYDROGENASES IN VARIOUS ORGANS OF THE RAT.

Authors: Y P LEE; H A LARDY
Journal: J Biol Chem Date: 1965-03 Impact factor: 5.157

3. Mono- and dimethylethanolamine isolated from rat-liver phospholipids.

Authors: J BREMER; D M GREENBERG
Journal: Biochim Biophys Acta Date: 1959-09

4. Liver choline oxidase activity in man and in several species of animals.

Authors: H SIDRANSKY; E FARBER
Journal: Arch Biochem Biophys Date: 1960-03 Impact factor: 4.013

5. Phosphatidylethanolamine in liver mitochondria and endoplasmic reticulum: molecular species distribution and turnover.

Authors: J G Parkes; W Thompson
Journal: Can J Biochem Date: 1975-06

6. Studies in vivo and in vitro on the methylation of phosphatidyl-N,N-dimethylethanolamine to phosphatidylcholine in rat liver.

Authors: D LeKim; H Betzing; W Stoffel
Journal: Hoppe Seylers Z Physiol Chem Date: 1973-04

7. Specificity for incorporation of choline and ethanolamine into rat-liver microsomal lecithins.

Authors: D Rytter; J E Miller; W E Cornatzer
Journal: Biochim Biophys Acta Date: 1968-03-04

8. Effect of diet on choline phosphotransferase, phosphatidylethanolamine methyltransferase and phosphatidyldimethylethanolamine methyltransferase in liver microsomes.

Authors: D R Hoffman; E O Uthus; W E Cornatzer
Journal: Lipids Date: 1980-06 Impact factor: 1.880

9. Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates.

Authors: R Sundler; B Akesson
Journal: J Biol Chem Date: 1975-05-10 Impact factor: 5.157

10. In vivo studies on pathways for the biosynthesis of lecithin in the rat.

Authors: P Bjørnstad; J Bremer
Journal: J Lipid Res Date: 1966-01 Impact factor: 5.922

9 in total

1. Beta-adrenergic control of phosphatidylcholine synthesis by transmethylation in hepatocytes from juvenile, adult and adrenalectomized rats.

Authors: D Marin-Cao; V Alvarez Chiva; J M Mato
Journal: Biochem J Date: 1983-12-15 Impact factor: 3.857

2. The effect of embryological development on phosphatidylethanolamine methyltransferase, phosphatidyldimethylethanolamine methyltransferase and choline phosphotransferase of rabbit liver microsomes.

Authors: W E Cornatzer; D R Hoffman; J A Haning
Journal: Lipids Date: 1984-01 Impact factor: 1.880

Microsomal phosphatidylethanolamine methyltransferase: some physical and kinetic properties.

1. RATES OF REACTIONS INVOLVED IN PHOSPHATIDE SYNTHESIS IN LIVER AND SMALL INTESTINE OF INTACT RATS.

2. INFLUENCE OF THYROID HORMONES ON L-ALPHA-GLYCEROPHOSPHATE DEHYDROGENASES AND OTHER DEHYDROGENASES IN VARIOUS ORGANS OF THE RAT.

3. Mono- and dimethylethanolamine isolated from rat-liver phospholipids.

4. Liver choline oxidase activity in man and in several species of animals.

5. Phosphatidylethanolamine in liver mitochondria and endoplasmic reticulum: molecular species distribution and turnover.

6. Studies in vivo and in vitro on the methylation of phosphatidyl-N,N-dimethylethanolamine to phosphatidylcholine in rat liver.

7. Specificity for incorporation of choline and ethanolamine into rat-liver microsomal lecithins.

8. Effect of diet on choline phosphotransferase, phosphatidylethanolamine methyltransferase and phosphatidyldimethylethanolamine methyltransferase in liver microsomes.

9. Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates.

10. In vivo studies on pathways for the biosynthesis of lecithin in the rat.

1. Beta-adrenergic control of phosphatidylcholine synthesis by transmethylation in hepatocytes from juvenile, adult and adrenalectomized rats.

2. The effect of embryological development on phosphatidylethanolamine methyltransferase, phosphatidyldimethylethanolamine methyltransferase and choline phosphotransferase of rabbit liver microsomes.

3. Influence of dietary fat on the lipid composition of rat brain synaptosomal and microsomal membranes.

4. Effect of pyridoxine deficiency on phospholipid methylation in rat liver microsomes.

5. Microsomal phosphatidylethanolamine methyltransferase: inhibition by S-adenosylhomocysteine.

6. Choline biosynthesis in sheep. Evidence for extrahepatic synthesis.

7. Effect of zinc deficiency on the biosynthesis of phosphatidylcholine in rat microsomes.

8. Calcium signals and phospholipid methylation in eukaryotic cells.

9. Purification and photoaffinity labelling of lipid methyltransferase from rat liver.