| Literature DB >> 7276125 |
S L Spruance, B N Ashton, C B Smith.
Abstract
A method is described to radiolabel measles virus RNA for hybridization studies. Tritiated nucleosides were added to the media of measles virus infected Vero cells and negative-strand (genome) RNA with a specific activity of 6 X 10(5) c.p.m./microgram was purified from viral nucleocapsids. 50 S RNA was the sole RNA present in nucleocapsids and self-annealed to 50% due to the presence of 25% 50 S plus-strands (anti-genomes).Entities:
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Year: 1980 PMID： 7276125 DOI： 10.1016/0166-0934(80)90062-2
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014