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Literature DB >> 7275691

The energy dispersive X-ray microanalysis technique in the study of fluids and tissues of the brain and the inner ear.

Abstract

The temporal bone from the fetal and adult mouse and brain from fetal and adult rat were cryosectioned in a conventional cryostat and -30 degrees C using 16 micrometers thick sections. The elemental composition was analyzed in the fluid-filled spaces of the membranous labyrinth and ventricular cavities of the brain. After drying these spaces containing small crystal-like aggregations of material which morphologically differed depending on their elemental composition. This changed during embryonic maturation. The qualitative X-ray analytical microscopy seems to be a reliable method to follow changes in the elemental composition of fluids.

Entities: Species

Mesh：

Year: 1981 PMID： 7275691 DOI： 10.1007/BF00517139

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

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References

21 in total

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Authors: B L Gupta; T A Hall; S H Maddrell; R B Moreton
Journal: Nature Date: 1976-11-18 Impact factor: 49.962

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Authors: T R Van de Water; R J Ruben
Journal: Acta Otolaryngol Date: 1971-04 Impact factor: 1.494

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Authors: G P Dempsey; S Bullivant
Journal: J Microsc Date: 1976-04 Impact factor: 1.758

4. Elemental composition of the developing inner ear.

Authors: M Anniko; R Wroblewski
Journal: Ann Otol Rhinol Laryngol Date: 1981 Jan-Feb Impact factor: 1.547

5. Preparation of biological material for X-ray microanalysis of diffusible elements. II. Comparison of different methods of drying ultrathin cryosections cut without a trough liquid.

Authors: T Barnard; L Sevéus
Journal: J Microsc Date: 1978-04 Impact factor: 1.758

6. Studies of ion distribution in the inner ear: scanning electron microscopy and x-ray microanalysis of freeze-dried cochlear specimens.

Authors: A F Ryan; M G Wickham; R C Bone
Journal: Hear Res Date: 1980-01 Impact factor: 3.208

The energy dispersive X-ray microanalysis technique in the study of fluids and tissues of the brain and the inner ear.

1. Distribution of ions in a fluid-transporting epithelium determined by electron-probe X-ray microanalysis.

2. Organ culture of the mammalian inner ear.

3. A copper block method for freezing non-cryoprotected tissue to produce ice-crystal-free regions for electron microscopy. I. Evaluation using freeze-substitution.

4. Elemental composition of the developing inner ear.

5. Preparation of biological material for X-ray microanalysis of diffusible elements. II. Comparison of different methods of drying ultrathin cryosections cut without a trough liquid.

6. Studies of ion distribution in the inner ear: scanning electron microscopy and x-ray microanalysis of freeze-dried cochlear specimens.

7. Application of cryosectioning to x-ray microanalysis of biological tissue.

8. Electron probe X-ray microanalysis of human muscle biopsies.

9. Electron probe analysis of vascular smooth muscle. Composition of mitochondria, nuclei, and cytoplasm.

10. The electrophysiological mapping of compartments within a mammalian cell.