Literature DB >> 7274387

A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and 3H-sucrose.

K Kageyama, T Yamashita, T Kobayashi, E Kimura.   

Abstract

A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26 +/- 0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood.

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Year:  1981        PMID： 7274387     DOI： 10.1007/BF01967959

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  7 in total

1.  Intercellular plasma of centrifuged human erythrocytes as measured by means of iodo 131 albumin.

Authors:  C A OWEN; M H POWER
Journal:  J Appl Physiol       Date:  1953-01       Impact factor: 3.531

2.  The distribution of sodium and potassium in oxygenated human blood and their effects upon the movements of water between cells and plasma.

Authors:  P M HALD; M TULIN
Journal:  Am J Physiol       Date:  1947-05

3.  Erythrocyte osmotic fragility and cell water--influence of pH and temperature.

Authors:  J R Murphy
Journal:  J Lab Clin Med       Date:  1969-08

4.  A new rapid method for measuring the water content of whole blood, plasma and packed red blood cells by gas chromatography.

Authors:  K Kageyama; T Okada; A Sarashi; Y Kinoshita; E Kimura
Journal:  Osaka City Med J       Date:  1977

5.  Effect of incubation on red cell shape and water content as measured by gas-liquid chromatography.

Authors:  K Kageyama
Journal:  Nouv Rev Fr Hematol       Date:  1979

6.  The transport of sodium into human erythrocytes in vivo.

Authors:  G L GOLD; A K SOLOMON
Journal:  J Gen Physiol       Date:  1955-01-20       Impact factor: 4.086

7.  OSMOTIC PROPERTIES OF HUMAN RED CELLS.

Authors:  D SAVITZ; V W SIDEL; A K SOLOMON
Journal:  J Gen Physiol       Date:  1964-09       Impact factor: 4.086
  7 in total
  1 in total

1.  Moderately controlled transport of ascorbate into aortic endothelial cells against slowdown of the cell cycle, decreasing of the concentration or increasing of coexistent glucose as compared with dehydroascorbate.

Authors:  Y Saitoh; N Nagao; R O'Uchida; T Yamane; K Kageyama; N Muto; N Miwa
Journal:  Mol Cell Biochem       Date:  1997-08       Impact factor: 3.396
  1 in total

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