Effect of 2,2'-diaminodiphenyldisulfide, a resin hardener, on rats.

Guided by structure-activity relationships among carcinogenic aromatic amines, a sulfur-containing aromatic diamine was designed as a possible noncarcinogenic replacement for the resin hardener 4,4'-methylenebis(2-chloroaniline) (MOCA). However, in small-scale tests in rats the analog diaminodiphenyldisulfide (DDDS) caused necrotic changes in the liver, acanthosis and hyperkeratosis of the forestomach, and atrophy and hyperplasia of the ductal epithelium of the kidney. One case of hepatocellular carcinoma was also noted. Furthermore, DDDS caused alterations in the hepatocellular carcinoma was also noted. Furthermore, DDDS caused alterations in the phospholipid pattern of liver, kidney, and spleen, increasing total phospholipid and cholesterol but decreasing triglyceride levels. However, it was not mutagenic in a Salmonella test system, even in the presence of S9 and microsomal fractions from Aroclor 1254-treated rats.